3UH3

HBI (L36V) CO bound

3UH3 の概要

• エントリーDOI: 10.2210/pdb3uh3/pdb
• 関連するPDBエントリー: 3SDH 3UGY 3UGZ 3UH5 3UH6 3UH7 3UHB 3UHC 3UHD 3UHE 3UHG 3UHH 3UHI 3UHK 3UHN 3UHQ 3UHR 3UHS 3UHT 3UHU 3UHV 3UHW 3UHX 3UHY 3UHZ 3UI0 4SDH
• 分子名称: Globin-1, PROTOPORPHYRIN IX CONTAINING FE, CARBON MONOXIDE, ... (4 entities in total)
• 機能のキーワード: allostery, oxygen binding, oxygen storage, oxygen transport
• 由来する生物種: Scapharca inaequivalvis (Ark clam)
• 細胞内の位置: Cytoplasm: P02213
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33195.55

構造登録者

Ren, Z.,Srajer, V.,Knapp, J.E.,Royer Jr., W.E. (登録日: 2011-11-03, 公開日: 2011-12-28, 最終更新日: 2023-09-13)

主引用文献

Ren, Z.,Srajer, V.,Knapp, J.E.,Royer, W.E.
Cooperative macromolecular device revealed by meta-analysis of static and time-resolved structures.
Proc.Natl.Acad.Sci.USA, 109:107-112, 2012

PubMed Abstract: Here we present a meta-analysis of a large collection of static structures of a protein in the Protein Data Bank in order to extract the progression of structural events during protein function. We apply this strategy to the homodimeric hemoglobin HbI from Scapharca inaequivalvis. We derive a simple dynamic model describing how binding of the first ligand in one of the two chemically identical subunits facilitates a second binding event in the other partner subunit. The results of our ultrafast time-resolved crystallographic studies support this model. We demonstrate that HbI functions like a homodimeric mechanical device, such as pliers or scissors. Ligand-induced motion originating in one subunit is transmitted to the other via conserved pivot points, where the E and F' helices from two partner subunits are "bolted" together to form a stable dimer interface permitting slight relative rotation but preventing sliding.

PubMed: 22171006
DOI: 10.1073/pnas.1109213108

実験手法

X-RAY DIFFRACTION (1.8 Å)