3UGM

Structure of TAL effector PthXo1 bound to its DNA target

3UGM の概要

• エントリーDOI: 10.2210/pdb3ugm/pdb
• 分子名称: TAL effector AvrBs3/PthA, DNA-1, DNA-2, ... (4 entities in total)
• 機能のキーワード: tal effector, transcription-dna complex, dna binding protein, transcription/dna
• 由来する生物種: Xanthomonas oryzae; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 132292.32

構造登録者

Mak, A.N.S.,Bradley, P.,Cernadas, R.A.,Bogdanove, A.J.,Stoddard, B.L. (登録日: 2011-11-02, 公開日: 2012-01-04, 最終更新日: 2024-02-28)

主引用文献

Mak, A.N.,Bradley, P.,Cernadas, R.A.,Bogdanove, A.J.,Stoddard, B.L.
The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target.
Science, 335:716-719, 2012

PubMed Abstract: DNA recognition by TAL effectors is mediated by tandem repeats, each 33 to 35 residues in length, that specify nucleotides via unique repeat-variable diresidues (RVDs). The crystal structure of PthXo1 bound to its DNA target was determined by high-throughput computational structure prediction and validated by heavy-atom derivatization. Each repeat forms a left-handed, two-helix bundle that presents an RVD-containing loop to the DNA. The repeats self-associate to form a right-handed superhelix wrapped around the DNA major groove. The first RVD residue forms a stabilizing contact with the protein backbone, while the second makes a base-specific contact to the DNA sense strand. Two degenerate amino-terminal repeats also interact with the DNA. Containing several RVDs and noncanonical associations, the structure illustrates the basis of TAL effector-DNA recognition.

PubMed: 22223736
DOI: 10.1126/science.1216211

実験手法

X-RAY DIFFRACTION (3 Å)