3UG0

Crystal structure of a Trp-less green fluorescent protein translated by the simplified genetic code

3UG0 の概要

• エントリーDOI: 10.2210/pdb3ug0/pdb
• 関連するPDBエントリー: 3UFZ
• 分子名称: Green fluorescent protein (2 entities in total)
• 機能のキーワード: green fluorescent protein, fluorescent protein
• 由来する生物種: Aequorea victoria
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25626.88

構造登録者

Kawahara-Kobayashi, A.,Araiso, Y.,Matsuda, T.,Yokoyama, S.,Kigawa, T.,Nureki, O.,Kiga, D. (登録日: 2011-11-02, 公開日: 2012-10-17, 最終更新日: 2024-11-20)

主引用文献

Kawahara-Kobayashi, A.,Masuda, A.,Araiso, Y.,Sakai, Y.,Kohda, A.,Uchiyama, M.,Asami, S.,Matsuda, T.,Ishitani, R.,Dohmae, N.,Yokoyama, S.,Kigawa, T.,Nureki, O.,Kiga, D.
Simplification of the genetic code: restricted diversity of genetically encoded amino acids.
Nucleic Acids Res., 40:10576-10584, 2012

PubMed Abstract: At earlier stages in the evolution of the universal genetic code, fewer than 20 amino acids were considered to be used. Although this notion is supported by a wide range of data, the actual existence and function of the genetic codes with a limited set of canonical amino acids have not been addressed experimentally, in contrast to the successful development of the expanded codes. Here, we constructed artificial genetic codes involving a reduced alphabet. In one of the codes, a tRNAAla variant with the Trp anticodon reassigns alanine to an unassigned UGG codon in the Escherichia coli S30 cell-free translation system lacking tryptophan. We confirmed that the efficiency and accuracy of protein synthesis by this Trp-lacking code were comparable to those by the universal genetic code, by an amino acid composition analysis, green fluorescent protein fluorescence measurements and the crystal structure determination. We also showed that another code, in which UGU/UGC codons are assigned to Ser, synthesizes an active enzyme. This method will provide not only new insights into primordial genetic codes, but also an essential protein engineering tool for the assessment of the early stages of protein evolution and for the improvement of pharmaceuticals.

PubMed: 22909996
DOI: 10.1093/nar/gks786

実験手法

X-RAY DIFFRACTION (2.093 Å)