3UEX

Bovine beta-lactoglobulin complex with stearic acid

3UEX の概要

• エントリーDOI: 10.2210/pdb3uex/pdb
• 関連するPDBエントリー: 3NPO 3NQ3 3NQ9 3QZJ 3QZK 3UEU 3UEV 3UEW
• 分子名称: Beta-lactoglobulin, STEARIC ACID, ETHANOL, ... (4 entities in total)
• 機能のキーワード: beta protein, beta barrel, lipocalin, bovine milk, transport protein
• 由来する生物種: Bos taurus (bovine,cow,domestic cattle,domestic cow)
• 細胞内の位置: Secreted: P02754
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18723.85

構造登録者

Loch, J.,Lewinski, K. (登録日: 2011-10-31, 公開日: 2011-11-09, 最終更新日: 2024-10-30)

主引用文献

Loch, J.I.,Polit, A.,Bonarek, P.,Olszewska, D.,Kurpiewska, K.,Dziedzicka-Wasylewska, M.,Lewinski, K.
Bovine beta-lactoglobulin complex with stearic acid
Int.J.Biol.Macromol., 50:1095-1102, 2012

PubMed Abstract: Lactoglobulin is a globular milk protein for which physiological function has not been clarified. Due to its binding properties lactoglobulin might serve as a carrier for bioactive molecules. Binding of 12-, 14-, 16- and 18-carbon saturated fatty acids to bovine β-lactoglobulin has been characterised by isothermal titration calorimetry and X-ray crystallography as a part of systematic studies of lactoglobulin complexes with ligands of biological importance. The thermodynamic parameters have been determined for lauric, myristic and palmitic acid complexes revealing systematic decrease of enthalpic and increase of entropic component of ΔG with elongation of aliphatic chain. In all crystal structures determined with resolution 1.9-2.1Å, single fatty acid molecule was found in the β-barrel in extended conformation with individual pattern of interactions. Location of a fatty acid in the binding site depends on the length of aliphatic chain and influences polar interactions between protein and ligand. Systematic changes of entropic component indicate important role of water in binding process.

PubMed: 22425630
DOI: 10.1016/j.ijbiomac.2012.03.002

実験手法

X-RAY DIFFRACTION (2.1 Å)