3UD8

Crystal Structure Analysis of FGF1-Disaccharide(NI22) complex

Summary for 3UD8

• Entry DOI: 10.2210/pdb3ud8/pdb
• Related: 3UD7 3UD9 3UDA
• Descriptor: Heparin-binding growth factor 1, 2-deoxy-3-O-sulfo-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-1-O-methyl-2-O-sulfo-alpha-L-idopyranuronic acid, PHOSPHATE ION, ... (4 entities in total)
• Functional Keywords: heparin/heparan sulfate binding, hormone
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 3
• Total formula weight: 48766.64

Authors

Hung, S.-C.,Shi, Z. (deposition date: 2011-10-27, release date: 2012-11-28, Last modification date: 2023-11-01)

Primary citation

Hu, Y.-P.,Zhong, Y.-Q.,Chen, Z.-G.,Chen, C.-Y.,Shi, Z.,Zulueta, M.M.L.,Ku, C.-C.,Lee, P.-Y.,Wang, C.-C.,Hung, S.-C.
Divergent synthesis of 48 heparan sulfate-based disaccharides and probing the specific sugar-fibroblast growth factor-1 interaction
J.Am.Chem.Soc., 134:20722-20727, 2012

PubMed Abstract: Several biological processes involve glycans, yet understanding their ligand specificities is impeded by their inherent diversity and difficult acquisition. Generating broad synthetic sugar libraries for bioevaluations is a powerful tool in unraveling glycan structural information. In the case of the widely distributed heparan sulfate (HS), however, the 48 theoretical possibilities for its repeating disaccharide call for synthetic approaches that should minimize the effort in an undoubtedly huge undertaking. Here we employed a divergent strategy to afford all 48 HS-based disaccharides from just two orthogonally protected disaccharide precursors. Different combinations and sequence of transformation steps were applied with many downstream intermediates leading up to multiple target products. With the full disaccharide library in hand, affinity screening with fibroblast growth factor-1 (FGF-1) revealed that four of the synthetic sugars bind to FGF-1. The molecular details of the interaction were further clarified through X-ray analysis of the sugar-protein cocrystals. The capability of comprehensive sugar libraries in providing key insights in glycan-ligand interaction is, thus, highlighted.

PubMed: 23240683
DOI: 10.1021/ja3090065

Experimental method

X-RAY DIFFRACTION (2.37 Å)