3UCE

Crystal structure of a small-chain dehydrogenase in complex with NADPH

3UCE の概要

• エントリーDOI: 10.2210/pdb3uce/pdb
• 関連するPDBエントリー: 3UCF
• 分子名称: Dehydrogenase, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (3 entities in total)
• 機能のキーワード: rossmann fold, small-chain dehydrogenase, oxidoreductase
• 由来する生物種: Vibrio vulnificus
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97705.58

構造登録者

Buysschaert, G.,Verstraete, K.,Savvides, S.,Vergauwen, B. (登録日: 2011-10-26, 公開日: 2012-11-28, 最終更新日: 2023-09-13)

主引用文献

Buysschaert, G.,Verstraete, K.,Savvides, S.N.,Vergauwen, B.
Structural and biochemical characterization of an atypical short-chain dehydrogenase/reductase reveals an unusual cofactor preference.
Febs J., 280:1358-1370, 2013

PubMed Abstract: Short-chain dehydrogenases/reductases (SDRs) encompass a large and functionally diverse family of enzymes with representative members in all kingdoms of life. Despite the wealth of reactions catalyzed by SDRs, they operate through a well-conserved and efficient reaction mechanism centered in a conserved catalytic tetrad (Asn-Ser-Tyr-Lys) and the employment of an appropriate cofactor. In recent years, SDRs that lack the signature catalytic tetrad have been identified, thus adding a perplexing twist to SDR functionality. In the present study, we report the crystal structure of SDRvv, an atypical SDR from Vibrio vulnificus devoid of the catalytic tetrad, thereby defining the structural signature of this apparent SDR family outlier. Further structural analysis of SDRvv in complex with its putative cofactor NADPH, site-directed mutagenesis and binding studies via isothermal titration calorimetry, and further biochemical characterization have allowed us to dissect the cofactor preferences of SDRvv. The retained capacity to bind the NADPH cofactor, the conceivable existence of a proton relay and the conservation of the coordination distances between the key residues in the cofactor binding pocket define a first set of rules towards catalytic activity for SDRvv. The findings of the present study set the stage for deriving the identity of the natural substrate of SDRvv and add a new twist to the structure-function landscape for Rossmann-fold-dependent cofactor discrimination.

PubMed: 23311896
DOI: 10.1111/febs.12128

実験手法

X-RAY DIFFRACTION (1.798 Å)