3UBF

Crystal structure of Drosophila N-cadherin EC1-3, I

3UBF の概要

• エントリーDOI: 10.2210/pdb3ubf/pdb
• 関連するPDBエントリー: 3UBG 3UBH
• 分子名称: Neural-cadherin, ZINC ION, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: cadherin, cell adhesion
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37148.50

構造登録者

Jin, X.,Walker, M.A.,Shapiro, L. (登録日: 2011-10-24, 公開日: 2011-12-21, 最終更新日: 2024-02-28)

主引用文献

Jin, X.,Walker, M.A.,Felsovalyi, K.,Vendome, J.,Bahna, F.,Mannepalli, S.,Cosmanescu, F.,Ahlsen, G.,Honig, B.,Shapiro, L.
Crystal structures of Drosophila N-cadherin ectodomain regions reveal a widely used class of Ca2+-free interdomain linkers.
Proc.Natl.Acad.Sci.USA, 109:E127-E134, 2012

PubMed Abstract: Vertebrate classical cadherins mediate selective calcium-dependent cell adhesion by mechanisms now understood at the atomic level. However, structures and adhesion mechanisms of cadherins from invertebrates, which are highly divergent yet function in similar roles, remain unknown. Here we present crystal structures of three- and four-tandem extracellular cadherin (EC) domain segments from Drosophila N-cadherin (DN-cadherin), each including the predicted N-terminal EC1 domain (denoted EC1') of the mature protein. While the linker regions for the EC1'-EC2' and EC3'-EC4' pairs display binding of three Ca(2+) ions similar to that of vertebrate cadherins, domains EC2' and EC3' are joined in a "kinked" orientation by a previously uncharacterized Ca(2+)-free linker. Biophysical analysis demonstrates that a construct containing the predicted N-terminal nine EC domains of DN-cadherin forms homodimers with affinity similar to vertebrate classical cadherins, whereas deleting the ninth EC domain ablates dimerization. These results suggest that, unlike their vertebrate counterparts, invertebrate cadherins may utilize multiple EC domains to form intercellular adhesive bonds. Sequence analysis reveals that similar Ca(2+)-free linkers are widely distributed in the ectodomains of both vertebrate and invertebrate cadherins.

PubMed: 22171007
DOI: 10.1073/pnas.1117538108

実験手法

X-RAY DIFFRACTION (2.5 Å)