3U55

Crystal structure (Type-2) of SAICAR synthetase from Pyrococcus horikoshii OT3

3U55 の概要

• エントリーDOI: 10.2210/pdb3u55/pdb
• 関連するPDBエントリー: 3U54
• 分子名称: Phosphoribosylaminoimidazole-succinocarboxamide synthase, SULFATE ION, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: saicar synthetase-like fold, ligase, atp-binding, phosphoribosylaminoimidazole-succinocarboxamide synthetase, purc, de novo purine biosynthesis, ligation of carboxy and amine groups
• 由来する生物種: Pyrococcus horikoshii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27731.23

構造登録者

Manjunath, K.,Kanaujia, S.P.,Kanagaraj, S.,Jeyakanthan, J.,Sekar, K. (登録日: 2011-10-11, 公開日: 2012-10-17, 最終更新日: 2023-11-01)

主引用文献

Manjunath, K.,Kanaujia, S.P.,Kanagaraj, S.,Jeyakanthan, J.,Sekar, K.
Structure of SAICAR synthetase from Pyrococcus horikoshii OT3: insights into thermal stability
Int.J.Biol.Macromol., 53:7-19, 2013

PubMed Abstract: The first native crystal structure of Phosphoribosylaminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) from a hyperthermophilic organism Pyrococcus horikoshii OT3 was determined in two space groups H3 (Type-1: Resolution 2.35Å) and in C222(1) (Type-2: Resolution 1.9Å). Both are dimeric but Type-1 structure exhibited hexameric arrangement due to the presence of cadmium ions. A comparison has been made on the sequence and structures of all SAICAR synthetases to better understand the differences between mesophilic, thermophilic and hyperthermophilic SAICAR synthetases. These SAICAR synthetases are reasonably similar in sequence and three-dimensional structure; however, differences were visible only in the subtler details of percentage composition of the sequences, salt bridge interactions and non-polar contact areas.

PubMed: 23137517
DOI: 10.1016/j.ijbiomac.2012.10.028

実験手法

X-RAY DIFFRACTION (1.9 Å)