3U4K

Crystal structure of the receptor binding domain of plasmid-born adhesin MrkD1P of Klebsiella pneumoniae

3U4K の概要

• エントリーDOI: 10.2210/pdb3u4k/pdb
• 分子名称: Fimbria adhesin protein (1 entity in total)
• 機能のキーワード: pili adhesion, collagen v binder, pathogenicity, jelly-roll beta barrel fold, cell adhesion
• 由来する生物種: Klebsiella pneumoniae
• 細胞内の位置: Fimbrium: P21648
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19452.29

構造登録者

Rego, A.T.,Waksman, G. (登録日: 2011-10-09, 公開日: 2012-09-26, 最終更新日: 2024-11-20)

主引用文献

Rego, A.T.,Johnson, J.G.,Geibel, S.,Enguita, F.J.,Clegg, S.,Waksman, G.
Crystal structure of the MrkD1P receptor binding domain of Klebsiella pneumoniae and identification of the human collagen V binding interface.
Mol.Microbiol., 86:882-893, 2012

PubMed Abstract: Klebsiella species are members of the family enterobacteriaceae, opportunistic pathogens that are among the eight most prevalent infectious agents in hospitals. Among other virulence factors in Klebsiella, type 3 pili exhibit a unique binding pattern in the human kidney via interaction of two MrkD adhesion variants 1C1 and 1P to type IV and/or V collagen. However, very little is known about the nature of this recognition. Here we present the crystal structure of the plasmid born MrkD1P receptor domain (MrkDrd). The structure reveals a jelly-roll β-barrel fold comprising 17 β-strands very similar to the receptor domain of GafD, the tip adhesin from the F17 pilus that recognizes n-acetyl-d-glucosamine (GlcNAc). Analysis of collagen V binding of different MrkD1P mutants revealed that two regions were responsible for its binding: a pocket, that aligns approximately with the GlcNAc binding pocket of GafD involving residues R105 and Y155, and a transversally oriented patch that spans strands β2a, β9b and β6 including residues V49, T52, V91, R102 and I136. Taken together, these data provide structural and functional insights on MrkD1P recognition of host cells, providing a tool for future development of rationally designed drugs with the prospect of blocking Klebsiella adhesion to collagen V.

PubMed: 22988966
DOI: 10.1111/mmi.12023

実験手法

X-RAY DIFFRACTION (3 Å)