3U4A

From soil to structure: a novel dimeric family 3-beta-glucosidase isolated from compost using metagenomic analysis

3U4A の概要

• エントリーDOI: 10.2210/pdb3u4a/pdb
• 関連するPDBエントリー: 3U48
• 関連するBIRD辞書のPRD_ID: PRD_900005
• 分子名称: JMB19063, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: tim barrel, 3-beta-glucosidase, hydrolase
• 由来する生物種: compost metagenome
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 172054.61

構造登録者

McAndrew, R.P.,Park, J.I.,Reindl, W.,Friedland, G.D.,D'haeseleer, P.,Northen, T.,Sale, K.L.,Simmons, B.A.,Adams, P.D. (登録日: 2011-10-07, 公開日: 2013-04-10, 最終更新日: 2024-11-20)

主引用文献

McAndrew, R.P.,Park, J.I.,Heins, R.A.,Reindl, W.,Friedland, G.D.,D'haeseleer, P.,Northen, T.,Sale, K.L.,Simmons, B.A.,Adams, P.D.
From soil to structure, a novel dimeric beta-glucosidase belonging to glycoside hydrolase family 3 isolated from compost using metagenomic analysis.
J.Biol.Chem., 288:14985-14992, 2013

PubMed Abstract: A recent metagenomic analysis sequenced a switchgrass-adapted compost community to identify enzymes from microorganisms that were specifically adapted to switchgrass under thermophilic conditions. These enzymes are being examined as part of the pretreatment process for the production of "second-generation" biofuels. Among the enzymes discovered was JMB19063, a novel three-domain β-glucosidase that belongs to the GH3 (glycoside hydrolase 3) family. Here, we report the structure of JMB19063 in complex with glucose and the catalytic variant D261N crystallized in the presence of cellopentaose. JMB19063 is first structure of a dimeric member of the GH3 family, and we demonstrate that dimerization is required for catalytic activity. Arg-587 and Phe-598 from the C-terminal domain of the opposing monomer are shown to interact with bound ligands in the D261N structure. Enzyme assays confirmed that these residues are absolutely essential for full catalytic activity.

PubMed: 23580647
DOI: 10.1074/jbc.M113.458356

実験手法

X-RAY DIFFRACTION (2.196 Å)