3U38

Intercalation of lambda-[Ru(phen)2(dppz)]2+ into d(CCGGTACCGG)2

3U38 の概要

• エントリーDOI: 10.2210/pdb3u38/pdb
• 関連するPDBエントリー: 3QF8 3QRN
• 分子名称: 5'-D(*CP*CP*GP*GP*TP*AP*CP*CP*GP*G)-3', Lambda-Ru(phen)2(dppz) complex, BARIUM ION, ... (4 entities in total)
• 機能のキーワード: b-dna, ruthenium, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4670.88

構造登録者

Cardin, C.J.,Hall, J.P.,Niyazi, H. (登録日: 2011-10-05, 公開日: 2012-07-04, 最終更新日: 2024-03-20)

主引用文献

Niyazi, H.,Hall, J.P.,O'Sullivan, K.,Winter, G.,Sorensen, T.,Kelly, J.M.,Cardin, C.J.
Crystal structures of lambda-[Ru(phen)2dppz]2+ with oligonucleotides containing TA/TA and AT/AT steps show two intercalation modes
Nat Chem, 4:621-628, 2012

PubMed Abstract: The ruthenium complex [Ru(phen)(2)(dppz)](2+) (where phen is phenanthroline and dppz dipyridophenazine is known as a 'light switch' complex because its luminescence in solution is significantly enhanced in the presence of DNA. This property is poised to serve in diagnostic and therapeutic applications, but its binding mode with DNA needs to be elucidated further. Here, we describe the crystal structures of the Λ enantiomer bound to two oligonucleotide duplexes. The dppz ligand intercalates symmetrically and perpendicularly from the minor groove of the d(CCGGTACCGG)(2) duplex at the central TA/TA step, but not at the central AT/AT step of d(CCGGATCCGG)(2). In both structures, however, a second ruthenium complex links the duplexes through the combination of a shallower angled intercalation into the C(1)C(2)/G(9)G(10) step at the end of the duplex, and semi-intercalation into the G(3)G(4) step of an adjacent duplex. The TA/TA specificity of the perpendicular intercalation arises from the packing of phenanthroline ligands against the adenosine residue.

PubMed: 22824893
DOI: 10.1038/nchem.1397

実験手法

X-RAY DIFFRACTION (2.13 Å)