3U1M
Structure of the mRNA splicing complex component Cwc2
Summary for 3U1M
| Entry DOI | 10.2210/pdb3u1m/pdb |
| Related | 3U1L |
| Descriptor | Pre-mRNA-splicing factor CWC2, ZINC ION (3 entities in total) |
| Functional Keywords | csmp, zinc finger, mrna splicing, splicing |
| Biological source | Saccharomyces cerevisiae (Baker's yeast) |
| Cellular location | Nucleus: Q12046 |
| Total number of polymer chains | 1 |
| Total formula weight | 27438.51 |
| Authors | |
| Primary citation | Lu, P.,Lu, G.,Yan, C.,Wang, L.,Li, W.,Yin, P. Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition Biochem.J., 441:591-597, 2012 Cited by PubMed Abstract: The Prp19-associated complex [NTC (nineteen complex)] plays a crucial role in intron removal during premature mRNA splicing in eukaryotes. Only one component of the NTC, Cwc2, is capable of binding RNA. In the present study we report the 1.9 Å (1 Å=0.1 nm) X-ray structure of the Cwc2 core domain, which is both necessary and sufficient for RNA binding. The Cwc2 core domain contains two sub-domains, a CCCH-type ZnF (zinc finger) and a RRM (RNA recognition motif). Unexpectedly, the ZnF domain and the RRM form a single folding unit, glued together by extensive hydrophobic interactions and hydrogen bonds. Structure-guided mutational analysis revealed that the intervening loop [known as the RB loop (RNA-binding loop)] between ZnF and RRM plays an essential role in RNA binding. In addition, a number of highly conserved positively charged residues on the β-strands of RRM make an important contribution to RNA binding. Intriguingly, these residues and a portion of the RB loop constitute an extended basic surface strip that encircles Cwc2 halfway. The present study serves as a framework for understanding the regulatory function of the NTC in RNA splicing. PubMed: 21957909DOI: 10.1042/BJ20111385 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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