3U1M

Structure of the mRNA splicing complex component Cwc2

3U1M の概要

• エントリーDOI: 10.2210/pdb3u1m/pdb
• 関連するPDBエントリー: 3U1L
• 分子名称: Pre-mRNA-splicing factor CWC2, ZINC ION (3 entities in total)
• 機能のキーワード: csmp, zinc finger, mrna splicing, splicing
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Nucleus: Q12046
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27438.51

構造登録者

Lu, P.,Lu, G.,Yan, C.,Wang, L.,Li, W.,Yin, P. (登録日: 2011-09-30, 公開日: 2011-11-16, 最終更新日: 2023-11-01)

主引用文献

Lu, P.,Lu, G.,Yan, C.,Wang, L.,Li, W.,Yin, P.
Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition
Biochem.J., 441:591-597, 2012

PubMed Abstract: The Prp19-associated complex [NTC (nineteen complex)] plays a crucial role in intron removal during premature mRNA splicing in eukaryotes. Only one component of the NTC, Cwc2, is capable of binding RNA. In the present study we report the 1.9 Å (1 Å=0.1 nm) X-ray structure of the Cwc2 core domain, which is both necessary and sufficient for RNA binding. The Cwc2 core domain contains two sub-domains, a CCCH-type ZnF (zinc finger) and a RRM (RNA recognition motif). Unexpectedly, the ZnF domain and the RRM form a single folding unit, glued together by extensive hydrophobic interactions and hydrogen bonds. Structure-guided mutational analysis revealed that the intervening loop [known as the RB loop (RNA-binding loop)] between ZnF and RRM plays an essential role in RNA binding. In addition, a number of highly conserved positively charged residues on the β-strands of RRM make an important contribution to RNA binding. Intriguingly, these residues and a portion of the RB loop constitute an extended basic surface strip that encircles Cwc2 halfway. The present study serves as a framework for understanding the regulatory function of the NTC in RNA splicing.

PubMed: 21957909
DOI: 10.1042/BJ20111385

実験手法

X-RAY DIFFRACTION (1.95 Å)