3TYF

Crystal structure of a CD1d-lysophosphatidylcholine reactive iNKT TCR

Summary for 3TYF

• Entry DOI: 10.2210/pdb3tyf/pdb
• Related: 3TZV 3U0P
• Descriptor: iNKT Cell Receptor Alpha Chain, iNKT Cell Receptor Beta Chain, GLYCEROL, ... (4 entities in total)
• Functional Keywords: immunoglobulin-like, antigen recognition, cd1d, membrane, immune system
• Biological source: Escherichia coli; More
• Total number of polymer chains: 4
• Total formula weight: 106391.97

Authors

Lopez-Sagaseta, J.,Adams, E.J. (deposition date: 2011-09-24, release date: 2012-04-25)

Primary citation

Lopez-Sagaseta, J.,Sibener, L.V.,Kung, J.E.,Gumperz, J.,Adams, E.J.
Lysophospholipid presentation by CD1d and recognition by a human Natural Killer T-cell receptor.
Embo J., 31:2047-2059, 2012

PubMed Abstract: Invariant Natural Killer T (iNKT) cells use highly restricted αβ T cell receptors (TCRs) to probe the repertoire of lipids presented by CD1d molecules. Here, we describe our studies of lysophosphatidylcholine (LPC) presentation by human CD1d and its recognition by a native, LPC-specific iNKT TCR. Human CD1d presenting LPC adopts an altered conformation from that of CD1d presenting glycolipid antigens, with a shifted α1 helix resulting in an open A' pocket. Binding of the iNKT TCR requires a 7-Å displacement of the LPC headgroup but stabilizes the CD1d-LPC complex in a closed conformation. The iNKT TCR CDR loop footprint on CD1d-LPC is anchored by the conserved positioning of the CDR3α loop, whereas the remaining CDR loops are shifted, due in part to amino-acid differences in the CDR3β and Jβ segment used by this iNKT TCR. These findings provide insight into how lysophospholipids are presented by human CD1d molecules and how this complex is recognized by some, but not all, human iNKT cells.

PubMed: 22395072
DOI: 10.1038/emboj.2012.54

Experimental method

X-RAY DIFFRACTION (2.806 Å)