3TTC

Crystal structure of E. coli HypF with ADP and carbamoyl phosphate

3TTC の概要

• エントリーDOI: 10.2210/pdb3ttc/pdb
• 関連するPDBエントリー: 3TSP 3TSQ 3TSU 3TTD 3TTF
• 分子名称: Transcriptional regulatory protein, ZINC ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: zn finger, nucleotide binding, hydrogenase maturation factor, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 72201.14

構造登録者

Petkun, S.,Shi, R.,Li, Y.,Cygler, M. (登録日: 2011-09-14, 公開日: 2011-12-28, 最終更新日: 2024-02-28)

主引用文献

Petkun, S.,Shi, R.,Li, Y.,Asinas, A.,Munger, C.,Zhang, L.,Waclawek, M.,Soboh, B.,Sawers, R.G.,Cygler, M.
Structure of Hydrogenase Maturation Protein HypF with Reaction Intermediates Shows Two Active Sites.
Structure, 19:1773-1783, 2011

PubMed Abstract: [NiFe]-hydrogenases are multimeric proteins. The large subunit contains the NiFe(CN)(2)CO bimetallic active center and the small subunit contains Fe-S clusters. Biosynthesis and assembly of the NiFe(CN)(2)CO active center requires six Hyp accessory proteins. The synthesis of the CN(-) ligands is catalyzed by the combined actions of HypF and HypE using carbamoylphosphate as a substrate. We report the structure of Escherichia coli HypF(92-750) lacking the N-terminal acylphosphatase domain. HypF(92-750) comprises the novel Zn-finger domain, the nucleotide-binding YrdC-like domain, and the Kae1-like universal domain, also binding a nucleotide and a Zn(2+) ion. The two nucleotide-binding sites are sequestered in an internal cavity, facing each other and separated by ∼14 Å. The YrdC-like domain converts carbamoyl moiety to a carbamoyl adenylate intermediate, which is channeled to the Kae1-like domain. Mutations within either nucleotide-binding site compromise hydrogenase maturation but do not affect the carbamoylphosphate phosphatase activity.

PubMed: 22153500
DOI: 10.1016/j.str.2011.09.023

実験手法

X-RAY DIFFRACTION (1.86 Å)