3TRZ

Mouse Lin28A in complex with let-7d microRNA pre-element

Summary for 3TRZ

• Entry DOI: 10.2210/pdb3trz/pdb
• Descriptor: Protein lin-28 homolog A, RNA (5'-R(*GP*GP*GP*CP*AP*GP*GP*GP*AP*UP*UP*UP*UP*GP*CP*CP*CP*GP*GP*AP*G)-3'), ZINC ION (3 entities in total)
• Functional Keywords: microrna biogenesis, protein-rna complex, pre-element, cchc zinc knuckle, cold shock domain, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Mus musculus (house mouse); More
• Cellular location: Cytoplasm: Q8K3Y3
• Total number of polymer chains: 12
• Total formula weight: 137745.23

Authors

Nam, Y.,Sliz, P. (deposition date: 2011-09-11, release date: 2011-11-16, Last modification date: 2024-02-28)

Primary citation

Nam, Y.,Chen, C.,Gregory, R.I.,Chou, J.J.,Sliz, P.
Molecular Basis for Interaction of let-7 MicroRNAs with Lin28.
Cell(Cambridge,Mass.), 147:1080-1091, 2011

PubMed Abstract: MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate gene expression. Among these, members of the let-7 miRNA family control many cell-fate determination genes to influence pluripotency, differentiation, and transformation. Lin28 is a specific, posttranscriptional inhibitor of let-7 biogenesis. We report crystal structures of mouse Lin28 in complex with sequences from let-7d, let-7-f1, and let-7 g precursors. The two folded domains of Lin28 recognize two distinct regions of the RNA and are sufficient for inhibition of let-7 in vivo. We also show by NMR spectroscopy that the linker connecting the two folded domains is flexible, accommodating Lin28 binding to diverse let-7 family members. Protein-RNA complex formation imposes specific conformations on both components that could affect downstream recognition by other processing factors. Our data provide a molecular explanation for Lin28 specificity and a model for how it regulates let-7.

PubMed: 22078496
DOI: 10.1016/j.cell.2011.10.020

Experimental method

X-RAY DIFFRACTION (2.9 Å)