3TPF

Crystal structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni subsp. jejuni NCTC 11168

3TPF の概要

• エントリーDOI: 10.2210/pdb3tpf/pdb
• 関連するPDBエントリー: 1C9Y
• 分子名称: Ornithine carbamoyltransferase, DI(HYDROXYETHYL)ETHER (3 entities in total)
• 機能のキーワード: structural genomics, center for structural genomics of infectious diseases, csgid, rossmann fold, transferase, carbamoyl phosphate binding, l-ornithine binding
• 由来する生物種: Campylobacter jejuni subsp. jejuni
• 細胞内の位置: Cytoplasm : Q9PNU6
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 214460.12

構造登録者

Shabalin, I.G.,Onopriyenko, O.,Grimshaw, S.,Porebski, P.J.,Grabowski, M.,Savchenko, A.,Chruszcz, M.,Anderson, W.F.,Minor, W.,Center for Structural Genomics of Infectious Diseases (CSGID) (登録日: 2011-09-07, 公開日: 2011-09-21, 最終更新日: 2024-11-20)

主引用文献

Shabalin, I.G.,Porebski, P.J.,Cooper, D.R.,Grabowski, M.,Onopriyenko, O.,Grimshaw, S.,Savchenko, A.,Chruszcz, M.,Minor, W.
Structure of anabolic ornithine carbamoyltransferase from Campylobacter jejuni at 2.7 A resolution.
Acta Crystallogr.,Sect.F, 68:1018-1024, 2012

PubMed Abstract: Anabolic ornithine transcarbamoylase (aOTC) catalyzes the reaction between carbamoyl phosphate (CP) and L-ornithine (ORN) to form L-citrulline and phosphate in the urea cycle and L-arginine biosynthesis. The crystal structure of unliganded aOTC from Campylobacter jejuni (Cje aOTC) was determined at 2.7 Å resolution and refined to an R(work) of 20.3% and an R(free) of 24.0%. Cje aOTC is a trimer that forms a head-to-head pseudohexamer in the asymmetric unit. Each monomer is composed of an N-terminal CP-binding domain and a C-terminal ORN-binding domain joined by two interdomain helices. The Cje aOTC structure presents an open conformation of the enzyme with a relatively flexible orientation of the ORN-binding domain respective to the CP-binding domain. The conformation of the B2-H3 loop (residues 68-78), which is involved in binding CP in an adjacent subunit of the trimer, differs from that seen in homologous proteins with CP bound. The loop containing the ORN-binding motif (DxxxSMG, residues 223-230) has a conformation that is different from those observed in unliganded OTC structures from other species, but is similar to those in structures with bound ORN analogs. The major differences in tertiary structure between Cje aOTC and human aOTC are described.

PubMed: 22949186
DOI: 10.1107/S1744309112031259

実験手法

X-RAY DIFFRACTION (2.7 Å)