3TOL

Crystal structure of an engineered cytochrome cb562 that forms 1D, Zn-mediated coordination polymers

3TOL の概要

• エントリーDOI: 10.2210/pdb3tol/pdb
• 関連するPDBエントリー: 2BC5 2QLA 3TOM
• 分子名称: Soluble cytochrome b562, PROTOPORPHYRIN IX CONTAINING FE, ZINC ION, ... (5 entities in total)
• 機能のキーワード: four helix bundle, electron transfer, periplasmic space, metal binding protein
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm: P0ABE7
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 49336.23

構造登録者

Brodin, J.B.,Tezcan, F.A. (登録日: 2011-09-05, 公開日: 2012-07-04, 最終更新日: 2024-02-28)

主引用文献

Brodin, J.D.,Ambroggio, X.I.,Tang, C.,Parent, K.N.,Baker, T.S.,Tezcan, F.A.
Metal-directed, chemically tunable assembly of one-, two- and three-dimensional crystalline protein arrays.
Nat Chem, 4:375-382, 2012

PubMed Abstract: Proteins represent the most sophisticated building blocks available to an organism and to the laboratory chemist. Yet, in contrast to nearly all other types of molecular building blocks, the designed self-assembly of proteins has largely been inaccessible because of the chemical and structural heterogeneity of protein surfaces. To circumvent the challenge of programming extensive non-covalent interactions to control protein self-assembly, we have previously exploited the directionality and strength of metal coordination interactions to guide the formation of closed, homoligomeric protein assemblies. Here, we extend this strategy to the generation of periodic protein arrays. We show that a monomeric protein with properly oriented coordination motifs on its surface can arrange, on metal binding, into one-dimensional nanotubes and two- or three-dimensional crystalline arrays with dimensions that collectively span nearly the entire nano- and micrometre scale. The assembly of these arrays is tuned predictably by external stimuli, such as metal concentration and pH.

PubMed: 22522257
DOI: 10.1038/nchem.1290

実験手法

X-RAY DIFFRACTION (2 Å)