3TNJ

Crystal structure of universal stress protein from Nitrosomonas europaea with AMP bound

3TNJ の概要

• エントリーDOI: 10.2210/pdb3tnj/pdb
• 関連するPDBエントリー: 2PFS
• 分子名称: Universal stress protein (Usp), ADENOSINE MONOPHOSPHATE (3 entities in total)
• 機能のキーワード: structural genomics, psi-biology, midwest center for structural genomics, mcsg, universal stress protein, chaperone
• 由来する生物種: Nitrosomonas europaea
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16963.09

構造登録者

Tkaczuk, K.L.,Chruszcz, M.,Shumilin, I.A.,Evdokimova, E.,Kagan, O.,Savchenko, A.,Edwards, A.,Joachimiak, A.,Minor, W.,Midwest Center for Structural Genomics (MCSG) (登録日: 2011-09-01, 公開日: 2011-09-14, 最終更新日: 2023-09-13)

主引用文献

Tkaczuk, K.L.,Shumilin, I.A.,Chruszcz, M.,Evdokimova, E.,Savchenko, A.,Minor, W.
Structural and functional insight into the universal stress protein family.
Evol Appl, 6:434-449, 2013

PubMed Abstract: We present the crystal structures of two universal stress proteins (USP) from Archaeoglobus fulgidus and Nitrosomonas europaea in both apo- and ligand-bound forms. This work is the first complete synthesis of the structural properties of 26 USP available in the Protein Data Bank, over 75% of which were determined by structure genomics centers with no additional information provided. The results of bioinformatic analyses of all available USP structures and their sequence homologs revealed that these two new USP structures share overall structural similarity with structures of USPs previously determined. Clustering and cladogram analyses, however, show how they diverge from other members of the USP superfamily and show greater similarity to USPs from organisms inhabiting extreme environments. We compared them with other archaeal and bacterial USPs and discuss their similarities and differences in context of structure, sequential motifs, and potential function. We also attempted to group all analyzed USPs into families, so that assignment of the potential function to those with no experimental data available would be possible by extrapolation.

PubMed: 23745136
DOI: 10.1111/eva.12057

実験手法

X-RAY DIFFRACTION (2 Å)