3TKK

Crystal Structure Analysis of a recombinant predicted acetamidase/ formamidase from the thermophile thermoanaerobacter tengcongensis

「3MJJ」から置き換えられました

3TKK の概要

• エントリーDOI: 10.2210/pdb3tkk/pdb
• 分子名称: Predicted acetamidase/formamidase, CALCIUM ION, ZINC ION, ... (4 entities in total)
• 機能のキーワード: beta/alpha structure, hydrolase
• 由来する生物種: Thermoanaerobacter tengcongensis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 129149.53

構造登録者

Qian, M.,Huang, Q.,Wu, G.,Lai, L.,Tang, Y.,Pei, J.,Kusunoki, M. (登録日: 2011-08-26, 公開日: 2011-11-16, 最終更新日: 2024-10-30)

主引用文献

Qian, M.,Huang, Q.,Wu, G.,Lai, L.,Tang, Y.,Pei, J.,Kusunoki, M.
Crystal Structure Analysis of a Recombinant Predicted Acetamidase/Formamidase from the Thermophile Thermoanaerobacter tengcongensis.
PROTEIN J., 31:166-174, 2012

PubMed Abstract: The structure of acetamidase/formamidase (Amds/Fmds) from the archaeon Thermoanaerobacter tengcongensis has been determined by X-ray diffraction analysis using MAD data in a crystal of space group P2₁, with unit-cell parameters a = 41.23 (3), b = 152.88 (6), c = 100.26 (7) Å, β = 99.49 (3) ° and been refined to a crystallographic R-factor of 17.4% and R-free of 23.7%. It contains two dimers in one asymmetric unit, in which native Amds/Fmds (TE19) contains of the 32 kDa native protein. The final model consists of 4 monomer (299 amino acids residues with additional 2 expression tag amino acids residues), 5 Ca²⁺, 4 Zn²⁺ and 853 water molecules. The monomer is composed by the following: an N-domain which is featuring by three-layers β/β/β; a prominent excursion between N-terminal end of strand β₇ and β₁₁, which contains four-stranded antiparallel β sheet; an C-domain which is formed by the last 82 amino acid residues with the feature of mixed α/β structure. The protein contains ion-pair Ca²⁺-Zn²⁺. The portion of three-layer β/β/β along with the loops provides four protein ligands to the tightly bound Ca²⁺, three water molecules complete the coordination; and provides five protein ligands to the tightly bound Zn²⁺, one water molecule complete the coordination.

PubMed: 22207484
DOI: 10.1007/s10930-011-9387-0

実験手法

X-RAY DIFFRACTION (1.99 Å)