3TJ1

Crystal Structure of RNA Polymerase I Transcription Initiation Factor Rrn3

3TJ1 の概要

• エントリーDOI: 10.2210/pdb3tj1/pdb
• 分子名称: RNA polymerase I-specific transcription initiation factor RRN3 (2 entities in total)
• 機能のキーワード: heat repeat, transcription factor, nucleus, transcription
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• 細胞内の位置: Nucleus : P36070
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 149663.77

構造登録者

Blattner, C.,Jennebach, S.,Herzog, F.,Mayer, A.,Cheung, A.C.M.,Witte, G.,Lorenzen, K.,Hopfner, K.-P.,Heck, A.J.R.,Aebersold, R.,Cramer, P. (登録日: 2011-08-23, 公開日: 2011-09-28, 最終更新日: 2024-02-28)

主引用文献

Blattner, C.,Jennebach, S.,Herzog, F.,Mayer, A.,Cheung, A.C.,Witte, G.,Lorenzen, K.,Hopfner, K.P.,Heck, A.J.,Aebersold, R.,Cramer, P.
Molecular basis of Rrn3-regulated RNA polymerase I initiation and cell growth.
Genes Dev., 25:2093-2105, 2011

PubMed Abstract: Cell growth is regulated during RNA polymerase (Pol) I transcription initiation by the conserved factor Rrn3/TIF-IA in yeast/humans. Here we provide a structure-function analysis of Rrn3 based on a combination of structural biology with in vivo and in vitro functional assays. The Rrn3 crystal structure reveals a unique HEAT repeat fold and a surface serine patch. Phosphorylation of this patch represses human Pol I transcription, and a phospho-mimetic patch mutation prevents Rrn3 binding to Pol I in vitro and reduces cell growth and Pol I gene occupancy in vivo. Cross-linking indicates that Rrn3 binds Pol I between its subcomplexes, AC40/19 and A14/43, which faces the serine patch. The corresponding region of Pol II binds the Mediator head that cooperates with transcription factor (TF) IIB. Consistent with this, the Rrn3-binding factor Rrn7 is predicted to be a TFIIB homolog. This reveals the molecular basis of Rrn3-regulated Pol I initiation and cell growth, and indicates a general architecture of eukaryotic transcription initiation complexes.

PubMed: 21940764
DOI: 10.1101/gad.17363311

実験手法

X-RAY DIFFRACTION (2.85 Å)