3THY

Human MutSbeta complexed with an IDL of 2 bases (Loop2) and ADP

3THY の概要

• エントリーDOI: 10.2210/pdb3thy/pdb
• 関連するPDBエントリー: 3THW 3THx 3THz
• 分子名称: DNA mismatch repair protein Msh2, DNA mismatch repair protein Msh3, DNA Loop2 minus strand, ... (6 entities in total)
• 機能のキーワード: abc family atpase, mismatch recognition, mismatched unpaired idl dna, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 224294.25

構造登録者

Yang, W. (登録日: 2011-08-19, 公開日: 2011-12-21, 最終更新日: 2024-02-28)

主引用文献

Gupta, S.,Gellert, M.,Yang, W.
Mechanism of mismatch repair revealed by human MutSbeta bound to unpaired DNA loops
Nat.Struct.Mol.Biol., 19:72-78, 2012

PubMed Abstract: DNA mismatch repair corrects replication errors, thus reducing mutation rates and microsatellite instability. Genetic defects in this pathway cause Lynch syndrome and various cancers in humans. Binding of a mispaired or unpaired base by bacterial MutS and eukaryotic MutSα is well characterized. We report here crystal structures of human MutSβ in complex with DNA containing insertion-deletion loops (IDL) of two, three, four or six unpaired nucleotides. In contrast to eukaryotic MutSα and bacterial MutS, which bind the base of a mismatched nucleotide, MutSβ binds three phosphates in an IDL. DNA is severely bent at the IDL; unpaired bases are flipped out into the major groove and partially exposed to solvent. A normal downstream base pair can become unpaired; a single unpaired base can thereby be converted to an IDL of two nucleotides and recognized by MutSβ. The C-terminal dimerization domains form an integral part of the MutS structure and coordinate asymmetrical ATP hydrolysis by Msh2 and Msh3 with mismatch binding to signal for repair.

PubMed: 22179786
DOI: 10.1038/nsmb.2175

実験手法

X-RAY DIFFRACTION (2.894 Å)