3TEF

Crystal Structure of the Periplasmic Catecholate-Siderophore Binding Protein VctP from Vibrio Cholerae

3TEF の概要

• エントリーDOI: 10.2210/pdb3tef/pdb
• 分子名称: Iron(III) ABC transporter, periplasmic iron-compound-binding protein (2 entities in total)
• 機能のキーワード: siderophore-binding protein, transport protein
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32638.67

構造登録者

Liu, X.,Wang, Z.,Liu, S.,Li, N.,Chen, Y.,Zhu, C.,Zhu, D.,Wei, T.,Huang, Y.,Xu, S.,Gu, L. (登録日: 2011-08-13, 公開日: 2012-08-15, 最終更新日: 2024-03-20)

主引用文献

Liu, X.,Du, Q.,Wang, Z.,Liu, S.,Li, N.,Chen, Y.,Zhu, C.,Zhu, D.,Wei, T.,Huang, Y.,Xu, S.,Gu, L.
Crystal structure of periplasmic catecholate-siderophore binding protein VctP from Vibrio cholerae at 1.7 A resolution
Febs Lett., 586:1240-1244, 2012

PubMed Abstract: VctP, one of the two essential siderophore-binding PBPs from the pathogen Vibrio cholerae, plays an important role in the transport of enterobactin and vibriobactin, which have quite different configurations of iron coordination, from the periplasm to the inner membrane. The current study reports the crystal structure of VctP from V. cholerae N16961 at 1.7Å resolution. A structural comparison of VctP with its homologues and the results of molecular docking indicate that enterobactin and vibriobactin share the same binding pocket. Significantly, a basic triad consisting of Arg137, Arg226 and Arg270 is used to balance the three negative charges of ferric-enterobactin, while a basic dyad consisting of Arg137 and Arg270 is used to balance the two negative charges of ferric-vibriobactin.

PubMed: 22575663
DOI: 10.1016/j.febslet.2012.03.043

実験手法

X-RAY DIFFRACTION (1.698 Å)