3TDL

Structure of human serum albumin in complex with DAUDA

3TDL の概要

• エントリーDOI: 10.2210/pdb3tdl/pdb
• 分子名称: Serum albumin, MYRISTIC ACID, 11-({[5-(dimethylamino)naphthalen-1-yl]sulfonyl}amino)undecanoic acid (3 entities in total)
• 機能のキーワード: protein-drug complex, human serum albumin, myristate, dauda, fluorescent fatty acid analogue, protein binding, transport protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P02768
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 68353.89

構造登録者

Wang, Y.,Luo, Z.,Shi, X.,Wang, H.,Nie, L. (登録日: 2011-08-11, 公開日: 2012-06-27, 最終更新日: 2024-11-13)

主引用文献

Wang, Y.,Luo, Z.,Shi, X.,Wang, H.,Nie, L.,Huang, M.
A fluorescent fatty acid probe, DAUDA, selectively displaces two myristates bound in human serum albumin
Protein Sci., 20:2095-2101, 2011

PubMed Abstract: 11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.

PubMed: 21997768
DOI: 10.1002/pro.749

実験手法

X-RAY DIFFRACTION (2.6 Å)