3TDH

Structure of the regulatory fragment of sccharomyces cerevisiae AMPK in complex with AMP

3TDH の概要

• エントリーDOI: 10.2210/pdb3tdh/pdb
• 関連するPDBエントリー: 3TE5
• 分子名称: Carbon catabolite-derepressing protein kinase, SNF1 protein kinase subunit beta-2, Nuclear protein SNF4, ... (5 entities in total)
• 機能のキーワード: cbs domain, nucleotide binding, cytosol, transferase
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Nucleus membrane; Peripheral membrane protein: P06782; Cytoplasm: P34164; Nucleus: P12904
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 70350.01

構造登録者

Mayer, F.V.,Heath, R.,Underwood, E.,Sanders, M.J.,Carmena, D.,McCartney, R.,Leiper, F.C.,Xiao, B.,Jing, C.,Walker, P.A.,Haire, L.F.,Ogrodowicz, R.,Martin, S.R.,Schmidt, M.C.,Gamblin, S.J.,Carling, D. (登録日: 2011-08-11, 公開日: 2011-11-09, 最終更新日: 2024-02-28)

主引用文献

Mayer, F.V.,Heath, R.,Underwood, E.,Sanders, M.J.,Carmena, D.,McCartney, R.R.,Leiper, F.C.,Xiao, B.,Jing, C.,Walker, P.A.,Haire, L.F.,Ogrodowicz, R.,Martin, S.R.,Schmidt, M.C.,Gamblin, S.J.,Carling, D.
ADP Regulates SNF1, the Saccharomyces cerevisiae Homolog of AMP-Activated Protein Kinase.
Cell Metab, 14:707-714, 2011

PubMed Abstract: The SNF1 protein kinase complex plays an essential role in regulating gene expression in response to the level of extracellular glucose in budding yeast. SNF1 shares structural and functional similarities with mammalian AMP-activated protein kinase. Both kinases are activated by phosphorylation on a threonine residue within the activation loop segment of the catalytic subunit. Here we show that ADP is the long-sought metabolite that activates SNF1 in response to glucose limitation by protecting the enzyme against dephosphorylation by Glc7, its physiologically relevant protein phosphatase. We also show that the regulatory subunit of SNF1 has two ADP binding sites. The tighter site binds AMP, ADP, and ATP competitively with NADH, whereas the weaker site does not bind NADH, but is responsible for mediating the protective effect of ADP on dephosphorylation. Mutagenesis experiments suggest that the general mechanism by which ADP protects against dephosphorylation is strongly conserved between SNF1 and AMPK.

PubMed: 22019086
DOI: 10.1016/j.cmet.2011.09.009

実験手法

X-RAY DIFFRACTION (2.3 Å)