3TC2
Crystal structure of potato serine protease inhibitor.
Summary for 3TC2
| Entry DOI | 10.2210/pdb3tc2/pdb |
| Descriptor | Kunitz-type proteinase inhibitor P1H5 (2 entities in total) |
| Functional Keywords | beta-trefoil fold, protease inhibitor, hydrolase inhibitor |
| Biological source | Solanum tuberosum (potatoes) |
| Total number of polymer chains | 3 |
| Total formula weight | 60971.65 |
| Authors | Meulenbroek, E.M.,Thomassen, E.A.J.,Pannu, N.S. (deposition date: 2011-08-08, release date: 2012-06-20, Last modification date: 2014-04-16) |
| Primary citation | Meulenbroek, E.M.,Thomassen, E.A.,Pouvreau, L.,Abrahams, J.P.,Gruppen, H.,Pannu, N.S. Structure of a post-translationally processed heterodimeric double-headed Kunitz-type serine protease inhibitor from potato. Acta Crystallogr.,Sect.D, 68:794-799, 2012 Cited by PubMed Abstract: Potato serine protease inhibitor (PSPI) constitutes about 22% of the total amount of proteins in potato tubers (cv. Elkana), making it the most abundant protease inhibitor in the plant. PSPI is a heterodimeric double-headed Kunitz-type serine protease inhibitor that can tightly and simultaneously bind two serine proteases by mimicking the substrate of the enzyme with its reactive-site loops. Here, the crystal structure of PSPI is reported, representing the first heterodimeric double-headed Kunitz-type serine protease inhibitor structure to be determined. PSPI has a β-trefoil fold and, based on the structure, two reactive-site loops bearing residues Phe75 and Lys95 were identified. PubMed: 22751664DOI: 10.1107/S090744491201222X PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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