3TC2

Crystal structure of potato serine protease inhibitor.

3TC2 の概要

• エントリーDOI: 10.2210/pdb3tc2/pdb
• 分子名称: Kunitz-type proteinase inhibitor P1H5 (2 entities in total)
• 機能のキーワード: beta-trefoil fold, protease inhibitor, hydrolase inhibitor
• 由来する生物種: Solanum tuberosum (potatoes)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60971.65

構造登録者

Meulenbroek, E.M.,Thomassen, E.A.J.,Pannu, N.S. (登録日: 2011-08-08, 公開日: 2012-06-20, 最終更新日: 2024-11-20)

主引用文献

Meulenbroek, E.M.,Thomassen, E.A.,Pouvreau, L.,Abrahams, J.P.,Gruppen, H.,Pannu, N.S.
Structure of a post-translationally processed heterodimeric double-headed Kunitz-type serine protease inhibitor from potato.
Acta Crystallogr.,Sect.D, 68:794-799, 2012

PubMed Abstract: Potato serine protease inhibitor (PSPI) constitutes about 22% of the total amount of proteins in potato tubers (cv. Elkana), making it the most abundant protease inhibitor in the plant. PSPI is a heterodimeric double-headed Kunitz-type serine protease inhibitor that can tightly and simultaneously bind two serine proteases by mimicking the substrate of the enzyme with its reactive-site loops. Here, the crystal structure of PSPI is reported, representing the first heterodimeric double-headed Kunitz-type serine protease inhibitor structure to be determined. PSPI has a β-trefoil fold and, based on the structure, two reactive-site loops bearing residues Phe75 and Lys95 were identified.

PubMed: 22751664
DOI: 10.1107/S090744491201222X

実験手法

X-RAY DIFFRACTION (1.6 Å)