3TB6

Structure of the effector-binding domain of arabinose repressor AraR from Bacillus subtilis

3TB6 の概要

• エントリーDOI: 10.2210/pdb3tb6/pdb
• 分子名称: Arabinose metabolism transcriptional repressor, beta-L-arabinopyranose, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: transcription regulation, arabinose binding, dna binding protein
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cytoplasm (Probable): P96711
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67264.99

構造登録者

Rezacova, P.,Prochazkova, K. (登録日: 2011-08-05, 公開日: 2012-01-25, 最終更新日: 2023-09-13)

主引用文献

Prochazkova, K.,Cermakova, K.,Pachl, P.,Sieglova, I.,Fabry, M.,Otwinowski, Z.,Rezacova, P.
Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis.
Acta Crystallogr.,Sect.D, 68:176-185, 2012

PubMed Abstract: In Bacillus subtilis, the arabinose repressor AraR negatively controls the expression of genes in the metabolic pathway of arabinose-containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: an N-terminal DNA-binding domain belonging to the GntR family and a C-terminal effector-binding domain that shows similarity to members of the GalR/LacI family. The crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose has been determined at 2.2 Å resolution. The L-arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry; the K(d) value was 8.4 ± 0.4 µM. The effect of L-arabinose on the protein oligomeric state was investigated in solution and detailed analysis of the crystal identified a dimer organization which is distinctive from that of other members of the GalR/LacI family.

PubMed: 22281747
DOI: 10.1107/S090744491105414X

実験手法

X-RAY DIFFRACTION (2.21 Å)