3TAY

Crystal structure of porcine rotavirus CRW-8 VP8* in complex with N-glycolylneuraminic acid

3TAY の概要

• エントリーDOI: 10.2210/pdb3tay/pdb
• 関連するPDBエントリー: 3TB0
• 分子名称: Outer capsid protein VP4, methyl 3,5-dideoxy-5-[(hydroxyacetyl)amino]-D-glycero-alpha-D-galacto-non-2-ulopyranosidonic acid, SULFATE ION, ... (8 entities in total)
• 機能のキーワード: beta sandwich, lectin, sugar binding protein, viral protein, neu5gc
• 由来する生物種: Porcine rotavirus (RV-A)
• 細胞内の位置: Outer capsid protein VP4: Virion. Outer capsid protein VP8*: Virion. Outer capsid protein VP5*: Virion: P0C6Y8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38646.81

構造登録者

Yu, X.,Blanchard, H. (登録日: 2011-08-04, 公開日: 2012-10-17, 最終更新日: 2024-02-28)

主引用文献

Yu, X.,Dang, V.T.,Fleming, F.E.,von Itzstein, M.,Coulson, B.S.,Blanchard, H.
Structural Basis of Rotavirus Strain Preference toward N-Acetyl- or N-Glycolylneuraminic Acid-Containing Receptors.
J.Virol., 86:13456-13466, 2012

PubMed Abstract: The rotavirus spike protein domain VP8* is essential for recognition of cell surface carbohydrate receptors, notably those incorporating N-acylneuraminic acids (members of the sialic acid family). N-Acetylneuraminic acids occur naturally in both animals and humans, whereas N-glycolylneuraminic acids are acquired only through dietary uptake in normal human tissues. The preference of animal rotaviruses for these natural N-acylneuraminic acids has not been comprehensively established, and detailed structural information regarding the interactions of different rotaviruses with N-glycolylneuraminic acids is lacking. In this study, distinct specificities of VP8* for N-acetyl- and N-glycolylneuraminic acids were revealed using biophysical techniques. VP8* protein from the porcine rotavirus CRW-8 and the bovine rotavirus Nebraska calf diarrhea virus (NCDV) showed a preference for N-glycolyl- over N-acetylneuraminic acids, in contrast to results obtained with rhesus rotavirus (RRV). Crystallographic structures of VP8* from CRW-8 and RRV with bound methyl-N-glycolylneuraminide revealed the atomic details of their interactions. We examined the influence of amino acid type at position 157, which is proximal to the ligand's N-acetyl or N-glycolyl moiety and can mutate upon cell culture adaptation. A structure-based hypothesis derived from these results could account for rotavirus discrimination between the N-acylneuraminic acid forms. Infectivity blockade experiments demonstrated that the determined carbohydrate specificities of these VP8* domains directly correlate with those of the corresponding infectious virus. This includes an association between CRW-8 adaption to cell culture, decreased competition by N-glycolylneuraminic acid for CRW-8 infectivity, and a Pro157-to-Ser157 mutation in VP8* that reduces binding affinity for N-glycolylneuraminic acid.

PubMed: 23035213
DOI: 10.1128/JVI.06975-11

実験手法

X-RAY DIFFRACTION (1.85 Å)