3TA2

A. fulgidus GlnK3, MgATP/2-OG complex

3TA2 の概要

• エントリーDOI: 10.2210/pdb3ta2/pdb
• 関連するPDBエントリー: 3T9Z 3TA0 3TA1
• 分子名称: Nitrogen regulatory protein P-II (GlnB-3), MAGNESIUM ION, 2-OXOGLUTARIC ACID, ... (6 entities in total)
• 機能のキーワード: pii-family, regulator, amt3, signaling protein
• 由来する生物種: Archaeoglobus fulgidus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 41642.84

構造登録者

Maier, S.,Schleberger, P.,Lue, W.,Wacker, T.,Pflueger, T.,Litz, C.,Andrade, S.L.A. (登録日: 2011-08-03, 公開日: 2011-10-26, 最終更新日: 2023-09-13)

主引用文献

Maier, S.,Schleberger, P.,Lu, W.,Wacker, T.,Pfluger, T.,Litz, C.,Andrade, S.L.
Mechanism of disruption of the Amt-GlnK complex by P(II)-mediated sensing of 2-oxoglutarate.
Plos One, 6:e26327-e26327, 2011

PubMed Abstract: GlnK proteins regulate the active uptake of ammonium by Amt transport proteins by inserting their regulatory T-loops into the transport channels of the Amt trimer and physically blocking substrate passage. They sense the cellular nitrogen status through 2-oxoglutarate, and the energy level of the cell by binding both ATP and ADP with different affinities. The hyperthermophilic euryarchaeon Archaeoglobus fulgidus possesses three Amt proteins, each encoded in an operon with a GlnK ortholog. One of these proteins, GlnK2 was recently found to be incapable of binding 2-OG, and in order to understand the implications of this finding we conducted a detailed structural and functional analysis of a second GlnK protein from A. fulgidus, GlnK3. Contrary to Af-GlnK2 this protein was able to bind both ATP/2-OG and ADP to yield inactive and functional states, respectively. Due to the thermostable nature of the protein we could observe the exact positioning of the notoriously flexible T-loops and explain the binding behavior of GlnK proteins to their interaction partner, the Amt proteins. A thermodynamic analysis of these binding events using microcalorimetry evaluated by microstate modeling revealed significant differences in binding cooperativity compared to other characterized P(II) proteins, underlining the diversity and adaptability of this class of regulatory signaling proteins.

PubMed: 22039461
DOI: 10.1371/journal.pone.0026327

実験手法

X-RAY DIFFRACTION (1.9 Å)