3T8R

Crystal structure of Staphylococcus aureus CymR

3T8R の概要

• エントリーDOI: 10.2210/pdb3t8r/pdb
• 関連するPDBエントリー: 3T8T
• 分子名称: Staphylococcus aureus CymR (2 entities in total)
• 機能のキーワード: transcriptional regulator protein, dimer, sulfenic acid, unknown function
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15932.23

構造登録者

He, C.,Ji, Q. (登録日: 2011-08-01, 公開日: 2012-05-16, 最終更新日: 2024-02-28)

主引用文献

Ji, Q.,Zhang, L.,Sun, F.,Deng, X.,Liang, H.,Bae, T.,He, C.
Staphylococcus aureus CymR Is a New Thiol-based Oxidation-sensing Regulator of Stress Resistance and Oxidative Response.
J.Biol.Chem., 287:21102-21109, 2012

PubMed Abstract: As a human pathogen, Staphylococcus aureus must cope with oxidative stress generated by the human immune system. Here, we report that CymR utilizes its sole Cys-25 to sense oxidative stress. Oxidation followed by thiolation of this cysteine residue leads to dissociation of CymR from its cognate promoter DNA. In contrast, the DNA binding of the CymRC25S mutant was insensitive to oxidation and thiolation, suggesting that CymR senses oxidative stress through oxidation of its sole cysteine to form a mixed disulfide with low molecular weight thiols. The determined crystal structures of the reduced and oxidized forms of CymR revealed that Cys-25 is oxidized to Cys-25-SOH in the presence of H(2)O(2). Deletion of cymR reduced the resistance of S. aureus to oxidative stresses, and the resistance was restored by expressing a C25S mutant copy of cymR. In a C25S substitution mutant, the expression of two genes, tcyP and mccB, was constitutively repressed and did not respond to hydrogen peroxide stress, whereas the expression of the genes were highly induced under oxidative stress in a wild-type strain, indicating the critical role of Cys-25 in redox signaling in vivo. Thus, CymR is another master regulator that senses oxidative stress and connects stress responses to virulence regulation in S. aureus.

PubMed: 22553203
DOI: 10.1074/jbc.M112.359737

実験手法

X-RAY DIFFRACTION (1.7 Å)