3T7U

A NeW Crystal structure of APC-ARM

3T7U の概要

• エントリーDOI: 10.2210/pdb3t7u/pdb
• 分子名称: Adenomatous polyposis coli protein, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: armadillo repeats domain, cell adhesion
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell junction, adherens junction : P25054
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84286.57

構造登録者

Zhang, Z.,Wu, G. (登録日: 2011-07-31, 公開日: 2011-12-14, 最終更新日: 2023-11-01)

主引用文献

Zhang, Z.,Lin, K.,Gao, L.,Chen, L.,Shi, X.,Wu, G.
Crystal structure of the armadillo repeat domain of adenomatous polyposis coli which reveals its inherent flexibility
Biochem.Biophys.Res.Commun., 412:732-736, 2011

PubMed Abstract: The conserved armadillo repeat (ARM) domain of adenomatous polyposis coli (APC) protein plays an important role in the recognition of its binding partners. In this study, we report the crystal structure of APC-ARM (residues 407-775), which was determined to 2.9 Å resolution. Our structure shows that the seven armadillo repeats of APC-ARM fold together into a compact domain, with Arm2 and Arm5 presenting some deviations from canonical armadillo repeats. There is a positively charged groove on the surface of APC-ARM, which might be the recognition site for APC-binding partners. Comparison of this structure with our previously reported structure of APC (407-751), together with normal mode analysis, reveals that the APC-ARM domain possesses a limited intrinsic flexibility. We propose that this intrinsic flexibility might be an inherent property of ARM domains in general.

PubMed: 21871439
DOI: 10.1016/j.bbrc.2011.08.044

実験手法

X-RAY DIFFRACTION (2.9 Å)