3T6W

Crystal Structure of Steccherinum ochraceum Laccase obtained by multi-crystals composite data collection technique (10% dose)

3T6W の概要

• エントリーDOI: 10.2210/pdb3t6w/pdb
• 関連するPDBエントリー: 3T6V 3T6X 3T6Z 3T71
• 分子名称: Laccase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, COPPER (II) ION, ... (8 entities in total)
• 機能のキーワード: beta barrel, oxidoreductase
• 由来する生物種: STECCHERINUM OCHRACEUM
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 163098.89

構造登録者

Ferraroni, M.,Briganti, F.,Matera, I.,Kolomytseva, M.,Golovleva, L.,Scozzafava, A.,Chernykh, A.M. (登録日: 2011-07-29, 公開日: 2012-04-18, 最終更新日: 2023-09-13)

主引用文献

Ferraroni, M.,Matera, I.,Chernykh, A.,Kolomytseva, M.,Golovleva, L.A.,Scozzafava, A.,Briganti, F.
Reaction intermediates and redox state changes in a blue laccase from Steccherinum ochraceum observed by crystallographic high/low X-ray dose experiments.
J.Inorg.Biochem., 111:203-209, 2012

PubMed Abstract: The crystal structure of a blue laccase from Steccherinum ochraceum has been solved at 2.0Å of resolution using a classic data acquisition from a single crystal. The overall structural features are typical of this class of enzymes, however, distances inside the trinuclear copper cluster are indicative of a reduction of the metal centers induced by free electrons produced during the X-ray data collection. UV-visible spectra collected during the X-ray exposure support the progressive reduction of the metal centers. In order to better detect the reduction progression steps in the trinuclear copper site, a multicrystal data collection strategy based on a systematic spread of the X-ray dose over many crystals has been employed. This approach is based on collecting multicrystal data sets, then combining the slices of the individual data sets experiencing the same radiation dose to obtain composite complete data sets at progressively higher doses. Applying this technique, we have been able to capture sequential frames of the enzyme during the metal centers and molecular oxygen reduction mechanism obtaining a three-dimensional movie of the X-ray-driven catalytic conversion of the molecular oxygen in the active site of laccase: first, the copper ions reduction, then the molecular oxygen binding and its reductive splitting, thus allowing to reconstruct the entire catalytic cycle for multicopper oxidases.

PubMed: 22341982
DOI: 10.1016/j.jinorgbio.2012.01.011

実験手法

X-RAY DIFFRACTION (2.15 Å)