3T6D

Crystal Structure of the Reaction Centre from Blastochloris viridis strain DSM 133 (ATCC 19567) substrain-08

3T6D の概要

• エントリーDOI: 10.2210/pdb3t6d/pdb
• 関連するPDBエントリー: 1DXR 1PRC 1VRN 2I5N 2JBL 2WJM 2WJN 3T6E
• 分子名称: Photosynthetic reaction center cytochrome c subunit, GLYCEROL, BACTERIOCHLOROPHYLL B, ... (18 entities in total)
• 機能のキーワード: pigment-protein complex, electron transport, photosynthesis, photosynthetic reaction center, photosynthetic membranes, evolution, genetic drift
• 由来する生物種: Blastochloris viridis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 162381.91

構造登録者

Roszak, A.W.,Gardiner, A.T.,Isaacs, N.W.,Cogdell, R.J. (登録日: 2011-07-28, 公開日: 2011-11-23, 最終更新日: 2024-11-27)

主引用文献

Roszak, A.W.,Moulisova, V.,Reksodipuro, A.D.,Gardiner, A.T.,Fujii, R.,Hashimoto, H.,Isaacs, N.W.,Cogdell, R.J.
New insights into the structure of the reaction centre from Blastochloris viridis: evolution in the laboratory.
Biochem.J., 442:27-37, 2012

PubMed Abstract: Newly determined crystal structures of the photosynthetic RC (reaction centre) from two substrains of the non-sulfur purple bacterium Blastochloris viridis strain DSM 133, together with analysis of their gene sequences, has revealed intraspecies evolutionary changes over a period of 14 years. Over 100 point mutations were identified between these two substrains in the four genes encoding the protein subunits of the RC, of which approximately one-fifth resulted in a total of 16 amino acid changes. The most interesting difference was in the M subunit where the change from a leucine residue to glycine in the carotenoid-binding pocket allowed NS5 (1,2-dihydroneurosporene) to adopt a more sterically favoured conformation, similar to the carotenoid conformation found in other related RCs. The results of the present study, together with a high rate of mutations in laboratory bacterial cultures described recently, suggest that bacteria evolve faster than has been generally recognized. The possibility that amino acid changes occur within protein sequences, without exhibiting any immediately observable phenotype, should be taken into account in studies that involve long-term continuous growth of pure bacterial cultures. The Blc. viridis RC is often studied with sophisticated biophysical techniques and changes such as those described here may well affect their outcome. In other words, there is a danger that laboratory-to-laboratory variation could well be due to different groups not realising that they are actually working with slightly different proteins. A way around this problem is suggested.

PubMed: 22054235
DOI: 10.1042/BJ20111540

実験手法

X-RAY DIFFRACTION (1.95 Å)