3T1T

MglA bound to GDP in P1 tetrameric arrangement

Summary for 3T1T

• Entry DOI: 10.2210/pdb3t1t/pdb
• Related: 3T12 3T1O 3T1Q 3T1R 3T1S 3T1V 3T1X
• Descriptor: Gliding protein mglA, GUANOSINE-5'-DIPHOSPHATE (3 entities in total)
• Functional Keywords: g domain containg protein, bacterial gtpase, bacterial polarity, motility, alpha/beta protein, hydrolase
• Biological source: Thermus thermophilus
• Total number of polymer chains: 4
• Total formula weight: 90707.93

Authors

Miertzschke, M.,Vetter, I.R.,Koerner, C.,Wittinghofer, A. (deposition date: 2011-07-22, release date: 2011-08-31, Last modification date: 2024-11-06)

Primary citation

Miertzschke, M.,Koerner, C.,Vetter, I.R.,Keilberg, D.,Hot, E.,Leonardy, S.,Sogaard-Andersen, L.,Wittinghofer, A.
Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity.
Embo J., 30:4185-4197, 2011

PubMed Abstract: The bacterium Myxococcus xanthus uses a G protein cycle to dynamically regulate the leading/lagging pole polarity axis. The G protein MglA is regulated by its GTPase-activating protein (GAP) MglB, thus resembling Ras family proteins. Here, we show structurally and biochemically that MglA undergoes a dramatic, GDP-GTP-dependent conformational change involving a screw-type forward movement of the central β2-strand, never observed in any other G protein. This movement and complex formation with MglB repositions the conserved residues Arg53 and Gln82 into the active site. Residues required for catalysis are thus not provided by the GAP MglB, but by MglA itself. MglB is a Roadblock/LC7 protein and functions as a dimer to stimulate GTP hydrolysis in a 2:1 complex with MglA. In vivo analyses demonstrate that hydrolysis mutants abrogate Myxococcus' ability to regulate its polarity axis changing the reversal behaviour from stochastic to oscillatory and that both MglA GTPase activity and MglB GAP catalysis are essential for maintaining a proper polarity axis.

PubMed: 21847100
DOI: 10.1038/emboj.2011.291

Experimental method

X-RAY DIFFRACTION (1.9 Å)