3SW1

Structure of a full-length bacterial LOV protein

3SW1 の概要

• エントリーDOI: 10.2210/pdb3sw1/pdb
• 分子名称: Sensory box protein, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: light-oxygen-voltage, lov, pas, signaling protein
• 由来する生物種: Pseudomonas putida
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38148.35

構造登録者

Granzin, J.,Batra-Safferling, R.,Jaeger, K.-E.,Drepper, T.,Krauss, U. (登録日: 2011-07-13, 公開日: 2012-02-15, 最終更新日: 2023-09-13)

主引用文献

Circolone, F.,Granzin, J.,Jentzsch, K.,Drepper, T.,Jaeger, K.E.,Willbold, D.,Krauss, U.,Batra-Safferling, R.
Structural Basis for the Slow Dark Recovery of a Full-Length LOV Protein from Pseudomonas putida.
J.Mol.Biol., 417:362-374, 2012

PubMed Abstract: Blue-light photoreceptors containing light–oxygen–voltage (LOV) domains regulate a myriad of different physiological responses in both eukaryotes and prokaryotes. Their light sensitivity is intricately linked to the photochemistry of the non-covalently bound flavin mononucleotide (FMN) chromophore that forms a covalent adduct with a conserved cysteine residue in the LOV domain upon illumination with blue light. All LOV domains undergo the same primary photochemistry leading to adduct formation; however, considerable variation is found in the lifetime of the adduct state that varies from seconds to several hours. The molecular mechanism underlying this variation among the structurally conserved LOV protein family is not well understood. Here, we describe the structural characterization of PpSB1-LOV, a very slow cycling full-length LOV protein from the Gram-negative bacterium Pseudomonas putida KT2440. Its crystal structure reveals a novel dimer interface that is mediated by N- and C-terminal auxiliary structural elements and a unique cluster of four arginine residues coordinating with the FMN-phosphate moiety. Site-directed mutagenesis of two arginines (R61 and R66) in PpSB1-LOV resulted in acceleration of the dark recovery reaction approximately by a factor of 280. The presented structural and biochemical data suggest a direct link between structural features and the slow dark recovery observed for PpSB1-LOV. The overall structural arrangement of PpSB1-LOV, together with a complementary phylogenetic analysis, highlights a common ancestry of bacterial LOV photoreceptors and Per-ARNT-Sim chemosensors.

PubMed: 22326872
DOI: 10.1016/j.jmb.2012.01.056

実験手法

X-RAY DIFFRACTION (2.63 Å)