3SW0

Structure of the C-terminal region (modules 18-20) of complement regulator Factor H

3SW0 の概要

• エントリーDOI: 10.2210/pdb3sw0/pdb
• 関連するPDBエントリー: 3OXU
• 分子名称: Complement factor H, GLYCEROL, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: innate immune response, sushi domains, cofactor in the inactivation of c3b, c3b, human plasma, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P08603
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21886.62

構造登録者

Morgan, H.P.,Guariento, M.,Schmidt, C.Q.,Barlow, P.N.,Hannan, J.P. (登録日: 2011-07-13, 公開日: 2012-03-14, 最終更新日: 2024-11-20)

主引用文献

Morgan, H.P.,Mertens, H.D.,Guariento, M.,Schmidt, C.Q.,Soares, D.C.,Svergun, D.I.,Herbert, A.P.,Barlow, P.N.,Hannan, J.P.
Structural Analysis of the C-Terminal Region (Modules 18-20) of Complement Regulator Factor H (FH).
Plos One, 7:e32187-e32187, 2012

PubMed Abstract: Factor H (FH) is a soluble regulator of the human complement system affording protection to host tissues. It selectively inhibits amplification of C3b, the activation-specific fragment of the abundant complement component C3, in fluid phase and on self-surfaces and accelerates the decay of the alternative pathway C3 convertase, C3bBb. We have determined the crystal structure of the three carboxyl-terminal complement control protein (CCP) modules of FH (FH18-20) that bind to C3b, and which additionally recognize polyanionic markers specific to self-surfaces. These CCPs harbour nearly 30 disease-linked missense mutations. We have also deployed small-angle X-ray scattering (SAXS) to investigate FH18-20 flexibility in solution using FH18-20 and FH19-20 constructs. In the crystal lattice FH18-20 adopts a "J"-shape: A ~122-degree tilt between the structurally highly similar modules 18 and 19 precedes an extended, linear arrangement of modules 19 and 20 as observed in previously determined structures of these two modules alone. However, under solution conditions FH18-20 adopts multiple conformations mediated by flexibility between CCPs 18 and 19. We also pinpoint the locations of disease-associated missense mutations on the module 18 surface and discuss our data in the context of the C3b:FH interaction.

PubMed: 22389686
DOI: 10.1371/journal.pone.0032187

実験手法

X-RAY DIFFRACTION (1.8 Å)