3ST3

Dreiklang - off state

3ST3 の概要

• エントリーDOI: 10.2210/pdb3st3/pdb
• 関連するPDBエントリー: 1HUY 3ST2 3ST4
• 分子名称: Dreiklang, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: gfp-like, beta barrel, reversibly switchable fluorescent protein, anthozoa, fluorescent dyes, luminescent protein, fluorescent protein
• 由来する生物種: Aequorea victoria
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 86909.41

構造登録者

Brakemann, T.,Weber, G.,Andresen, M.,Stiel, A.C.,Jakobs, S.,Wahl, M.C. (登録日: 2011-07-08, 公開日: 2011-09-14, 最終更新日: 2024-10-30)

主引用文献

Brakemann, T.,Stiel, A.C.,Weber, G.,Andresen, M.,Testa, I.,Grotjohann, T.,Leutenegger, M.,Plessmann, U.,Urlaub, H.,Eggeling, C.,Wahl, M.C.,Hell, S.W.,Jakobs, S.
A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching.
Nat.Biotechnol., 29:942-947, 2011

PubMed Abstract: Photoswitchable fluorescent proteins have enabled new approaches for imaging cells, but their utility has been limited either because they cannot be switched repeatedly or because the wavelengths for switching and fluorescence imaging are strictly coupled. We report a bright, monomeric, reversibly photoswitchable variant of GFP, Dreiklang, whose fluorescence excitation spectrum is decoupled from that for optical switching. Reversible on-and-off switching in living cells is accomplished at illumination wavelengths of ∼365 nm and ∼405 nm, respectively, whereas fluorescence is elicited at ∼515 nm. Mass spectrometry and high-resolution crystallographic analysis of the same protein crystal in the photoswitched on- and off-states demonstrate that switching is based on a reversible hydration/dehydration reaction that modifies the chromophore. The switching properties of Dreiklang enable far-field fluorescence nanoscopy in living mammalian cells using both a coordinate-targeted and a stochastic single molecule switching approach.

PubMed: 21909082
DOI: 10.1038/nbt.1952

実験手法

X-RAY DIFFRACTION (1.702 Å)