3SPU

apo NDM-1 Crystal Structure

3SPU の概要

• エントリーDOI: 10.2210/pdb3spu/pdb
• 分子名称: Beta-lactamase NDM-1, ZINC ION (3 entities in total)
• 機能のキーワード: alpha beta/beta alpha sandwich, hydrolase, beta lactam antibiotics, periplasmic space
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 141813.08

構造登録者

Strynadka, N.C.J.,King, D.T. (登録日: 2011-07-03, 公開日: 2011-08-03, 最終更新日: 2023-09-13)

主引用文献

King, D.,Strynadka, N.
Crystal structure of New Delhi metallo-beta-lactamase reveals molecular basis for antibiotic resistance
Protein Sci., 20:1484-1491, 2011

PubMed Abstract: β-Lactams are the most commonly prescribed class of antibiotics and have had an enormous impact on human health. Thus, it is disquieting that an enzyme called New Delhi metallo-β-lactamase-1 (NDM-1) can confer Enterobacteriaceae with nearly complete resistance to all β-lactam antibiotics including the carbapenams. We have determined the crystal structure of Klebsiella pneumoniae apo-NDM-1 to 2.1-Å resolution. From the structure, we see that NDM-1 has an expansive active site with a unique electrostatic profile, which we propose leads to a broader substrate specificity. In addition, NDM-1 undergoes important conformational changes upon substrate binding. These changes have not been previously observed in metallo-β-lactamase enzymes and may have a direct influence on substrate recognition and catalysis.

PubMed: 21774017
DOI: 10.1002/pro.697

実験手法

X-RAY DIFFRACTION (2.1 Å)