3SPE

Crystal structure of the tail sheath protein protease resistant fragment from bacteriophage phiKZ

3SPE の概要

• エントリーDOI: 10.2210/pdb3spe/pdb
• 関連するPDBエントリー: 3HXL 3J0H 3J0I 3LML
• 分子名称: PHIKZ029, PHOSPHATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: structural protein
• 由来する生物種: Pseudomonas phage phiKZ
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65344.80

構造登録者

Aksyuk, A.A.,Kurochkina, L.P.,Fokine, A.,Mesyanzhinov, V.V.,Rossmann, M.G. (登録日: 2011-07-01, 公開日: 2011-12-14, 最終更新日: 2024-10-30)

主引用文献

Aksyuk, A.A.,Kurochkina, L.P.,Fokine, A.,Forouhar, F.,Mesyanzhinov, V.V.,Tong, L.,Rossmann, M.G.
Structural conservation of the myoviridae phage tail sheath protein fold.
Structure, 19:1885-1894, 2011

PubMed Abstract: Bacteriophage phiKZ is a giant phage that infects Pseudomonas aeruginosa, a human pathogen. The phiKZ virion consists of a 1450 Å diameter icosahedral head and a 2000 Å-long contractile tail. The structure of the whole virus was previously reported, showing that its tail organization in the extended state is similar to the well-studied Myovirus bacteriophage T4 tail. The crystal structure of a tail sheath protein fragment of phiKZ was determined to 2.4 Å resolution. Furthermore, crystal structures of two prophage tail sheath proteins were determined to 1.9 and 3.3 Å resolution. Despite low sequence identity between these proteins, all of these structures have a similar fold. The crystal structure of the phiKZ tail sheath protein has been fitted into cryo-electron-microscopy reconstructions of the extended tail sheath and of a polysheath. The structural rearrangement of the phiKZ tail sheath contraction was found to be similar to that of phage T4.

PubMed: 22153511
DOI: 10.1016/j.str.2011.09.012

実験手法

X-RAY DIFFRACTION (2.3996 Å)