3SLQ

Crystal structure of the 2'- Deoxyguanosine riboswitch bound to guanosine-5'-monophosphate

3SLQ の概要

• エントリーDOI: 10.2210/pdb3slq/pdb
• 関連するPDBエントリー: 3SKI 3SKL 3SKT 3SKW 3SKZ 3SLM
• 分子名称: RNA (68-MER), GUANOSINE-5'-MONOPHOSPHATE, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: three-way junction, riboswitch, 2'-deoxy guanosine, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45554.72

構造登録者

Pikovskaya, O.,Polonskaia, A.,Patel, D.J.,Serganov, A. (登録日: 2011-06-24, 公開日: 2011-08-17, 最終更新日: 2024-02-28)

主引用文献

Pikovskaya, O.,Polonskaia, A.,Patel, D.J.,Serganov, A.
Structural principles of nucleoside selectivity in a 2'-deoxyguanosine riboswitch.
Nat.Chem.Biol., 7:748-755, 2011

PubMed Abstract: Purine riboswitches have an essential role in genetic regulation of bacterial metabolism. This family includes the 2'-deoxyguanosine (dG) riboswitch, which is involved in feedback control of deoxyguanosine biosynthesis. To understand the principles that define dG selectivity, we determined crystal structures of the natural Mesoplasma florum riboswitch bound to cognate dG as well as to noncognate guanosine, deoxyguanosine monophosphate and guanosine monophosphate. Comparison with related purine riboswitch structures reveals that the dG riboswitch achieves its specificity through modification of key interactions involving the nucleobase and rearrangement of the ligand-binding pocket to accommodate the additional sugar moiety. In addition, we observe new conformational changes beyond the junctional binding pocket extending as far as peripheral loop-loop interactions. It appears that re-engineering riboswitch scaffolds will require consideration of selectivity features dispersed throughout the riboswitch tertiary fold, and structure-guided drug design efforts targeted to junctional RNA scaffolds need to be addressed within such an expanded framework.

PubMed: 21841796
DOI: 10.1038/nchembio.631

実験手法

X-RAY DIFFRACTION (2.5 Å)