3SKI

Crystal structure of the 2'- Deoxyguanosine riboswitch bound to 2'-deoxyguanosine

Summary for 3SKI

• Entry DOI: 10.2210/pdb3ski/pdb
• Related: 3SKI 3SKL 3SKR 3SKT 3SKW 3SKZ 3SLM 3SLQ
• Descriptor: RNA (68-MER), 2'-DEOXY-GUANOSINE, MAGNESIUM ION, ... (7 entities in total)
• Functional Keywords: three-way junction, riboswitch, deoxyguanosine, rna
• Total number of polymer chains: 2
• Total formula weight: 45396.08

Authors

Pikovskaya, O.,Polonskaia, A.,Patel, D.J.,Serganov, A. (deposition date: 2011-06-22, release date: 2011-08-17, Last modification date: 2024-02-28)

Primary citation

Pikovskaya, O.,Polonskaia, A.,Patel, D.J.,Serganov, A.
Structural principles of nucleoside selectivity in a 2'-deoxyguanosine riboswitch.
Nat.Chem.Biol., 7:748-755, 2011

PubMed Abstract: Purine riboswitches have an essential role in genetic regulation of bacterial metabolism. This family includes the 2'-deoxyguanosine (dG) riboswitch, which is involved in feedback control of deoxyguanosine biosynthesis. To understand the principles that define dG selectivity, we determined crystal structures of the natural Mesoplasma florum riboswitch bound to cognate dG as well as to noncognate guanosine, deoxyguanosine monophosphate and guanosine monophosphate. Comparison with related purine riboswitch structures reveals that the dG riboswitch achieves its specificity through modification of key interactions involving the nucleobase and rearrangement of the ligand-binding pocket to accommodate the additional sugar moiety. In addition, we observe new conformational changes beyond the junctional binding pocket extending as far as peripheral loop-loop interactions. It appears that re-engineering riboswitch scaffolds will require consideration of selectivity features dispersed throughout the riboswitch tertiary fold, and structure-guided drug design efforts targeted to junctional RNA scaffolds need to be addressed within such an expanded framework.

PubMed: 21841796
DOI: 10.1038/nchembio.631

Experimental method

X-RAY DIFFRACTION (2.3 Å)