3SH5

Calcium-bound Laminin G like domain 3 from human perlecan

3SH5 の概要

• エントリーDOI: 10.2210/pdb3sh5/pdb
• 関連するPDBエントリー: 3SH4
• 分子名称: LG3 peptide, CALCIUM ION (3 entities in total)
• 機能のキーワード: actin disassambly activity, integrin alpha 2 beta 1, metal binding protein
• 由来する生物種: Homo sapiens
• 細胞内の位置: Secreted, extracellular space, extracellular matrix, basement membrane: P98160
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20611.88

構造登録者

Van Le, B.,Kim, K.K. (登録日: 2011-06-16, 公開日: 2011-12-14, 最終更新日: 2024-10-16)

主引用文献

Van Le, B.,Kim, H.,Choi, J.,Kim, J.H.,Hahn, M.J.,Lee, C.,Kim, K.K.,Hwang, H.Y.
Crystal Structure of the LG3 Domain of Endorepellin, an Angiogenesis Inhibitor.
J.Mol.Biol., 414:231-242, 2011

PubMed Abstract: Endorepellin, the C-terminal region of perlecan, inhibits angiogenesis by disrupting actin cytoskeleton and focal adhesions. The C-terminal laminin-like globular domain (LG3) of endorepellin directs most of this antiangiogenic activity. To investigate the angiostatic mechanism and to identify structural determinants, we have solved crystal structures of the LG3 domain in both apo- and calcium-bound forms at resolutions of 1.5 Å and 2.8 Å, respectively. The conserved core has the jellyroll fold characteristic of LG domains. The calcium-induced structural changes seem very restricted, and the calcium binding site appears to be preformed, suggesting that the bound calcium ion, rather than structural rearrangements, contributes to antiangiogenesis. We have identified H4268 on the EF loop as a key residue for the biochemical function of LG3, since its mutation abolishes antiangiogenic activity, and mutant LG3 can no longer form a direct interaction with integrin. Taken together, we propose that these two distinct structural elements contribute to the angiostatic effect of endorepellin.

PubMed: 21996443
DOI: 10.1016/j.jmb.2011.09.048

実験手法

X-RAY DIFFRACTION (2.8 Å)