3S51

Structure of FANCI

3S51 の概要

• エントリーDOI: 10.2210/pdb3s51/pdb
• 関連するPDBエントリー: 3S4W 3S4Z
• 分子名称: Fanconi anemia group I protein homolog (1 entity in total)
• 機能のキーワード: dna repair, dna binding protein
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Nucleus (By similarity): Q8K368
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 588482.25

構造登録者

Pavletich, N.P. (登録日: 2011-05-20, 公開日: 2011-07-27, 最終更新日: 2024-02-28)

主引用文献

Joo, W.,Xu, G.,Persky, N.S.,Smogorzewska, A.,Rudge, D.G.,Buzovetsky, O.,Elledge, S.J.,Pavletich, N.P.
Structure of the FANCI-FANCD2 complex: insights into the Fanconi anemia DNA repair pathway.
Science, 333:312-316, 2011

PubMed Abstract: Fanconi anemia is a cancer predisposition syndrome caused by defects in the repair of DNA interstrand cross-links (ICLs). Central to this pathway is the Fanconi anemia I-Fanconi anemia D2 (FANCI-FANCD2) (ID) complex, which is activated by DNA damage-induced phosphorylation and monoubiquitination. The 3.4 angstrom crystal structure of the ~300 kilodalton ID complex reveals that monoubiquitination and regulatory phosphorylation sites map to the I-D interface, suggesting that they occur on monomeric proteins or an opened-up complex and that they may serve to stabilize I-D heterodimerization. The 7.8 angstrom electron-density map of FANCI-DNA crystals and in vitro data show that each protein has binding sites for both single- and double-stranded DNA, suggesting that the ID complex recognizes DNA structures that result from the encounter of replication forks with an ICL.

PubMed: 21764741
DOI: 10.1126/science.1205805

実験手法

X-RAY DIFFRACTION (3.3 Å)