3S3Z

Crystal Structure an Tandem Cyanovirin-N Dimer, CVN2L10

3S3Z の概要

• エントリーDOI: 10.2210/pdb3s3z/pdb
• 関連するPDBエントリー: 3S3Y
• 分子名称: Tandem Cyanovirin-N Dimer CVN2L10, SODIUM ION (3 entities in total)
• 機能のキーワード: cyanovirin-n, sugar-binding, gp120, engineered dimer, antiviral protein
• 由来する生物種: Nostoc ellipsosporum; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24045.19

構造登録者

Keeffe, J.R.,Bjorkman, P.J.,Mayo, S.L. (登録日: 2011-05-18, 公開日: 2011-08-03, 最終更新日: 2024-11-20)

主引用文献

Keeffe, J.R.,Gnanapragasam, P.N.,Gillespie, S.K.,Yong, J.,Bjorkman, P.J.,Mayo, S.L.
Designed oligomers of cyanovirin-N show enhanced HIV neutralization.
Proc.Natl.Acad.Sci.USA, 108:14079-14084, 2011

PubMed Abstract: Cyanovirin-N (CV-N) is a small, cyanobacterial lectin that neutralizes many enveloped viruses, including human immunodeficiency virus type I (HIV-1). This antiviral activity is attributed to two homologous carbohydrate binding sites that specifically bind high mannose glycosylation present on envelope glycoproteins such as HIV-1 gp120. We created obligate CV-N oligomers to determine whether increasing the number of binding sites has an effect on viral neutralization. A tandem repeat of two CV-N molecules (CVN(2)) increased HIV-1 neutralization activity by up to 18-fold compared to wild-type CV-N. In addition, the CVN(2) variants showed extensive cross-clade reactivity and were often more potent than broadly neutralizing anti-HIV antibodies. The improvement in activity and broad cross-strain HIV neutralization exhibited by these molecules holds promise for the future therapeutic utility of these and other engineered CV-N variants.

PubMed: 21799112
DOI: 10.1073/pnas.1108777108

実験手法

X-RAY DIFFRACTION (1.75 Å)