3S1J

Crystal structure of acetate-bound hell's gate globin I

Summary for 3S1J

• Entry DOI: 10.2210/pdb3s1j/pdb
• Related: 3S1I
• Descriptor: Hemoglobin-like flavoprotein, PROTOPORPHYRIN IX CONTAINING FE, ACETATE ION, ... (5 entities in total)
• Functional Keywords: globin, heme, acetate-bound, autoxidation, oxygen transport, oxygen storage
• Biological source: Methylacidiphilum infernorum (METHYLOKORUS INFERNORUM)
• Total number of polymer chains: 3
• Total formula weight: 50093.53

Authors

Teh, A.H.,Saito, J.A.,Baharuddin, A.,Tuckerman, J.R.,Newhouse, J.S.,Kanbe, M.,Newhouse, E.I.,Rahim, R.A.,Favier, F.,Didierjean, C.,Sousa, E.H.S.,Stott, M.B.,Dunfield, P.F.,Gonzalez, G.,Gilles-Gonzalez, M.A.,Najimudin, N.,Alam, M. (deposition date: 2011-05-15, release date: 2011-09-21, Last modification date: 2024-03-20)

Primary citation

Teh, A.H.,Saito, J.A.,Baharuddin, A.,Tuckerman, J.R.,Newhouse, J.S.,Kanbe, M.,Newhouse, E.I.,Rahim, R.A.,Favier, F.,Didierjean, C.,Sousa, E.H.S.,Stott, M.B.,Dunfield, P.F.,Gonzalez, G.,Gilles-Gonzalez, M.A.,Najimudin, N.,Alam, M.
Hell's Gate globin I: an acid and thermostable bacterial hemoglobin resembling mammalian neuroglobin
Febs Lett., 585:3250-3258, 2011

PubMed Abstract: Hell's Gate globin I (HGbI), a heme-containing protein structurally homologous to mammalian neuroglobins, has been identified from an acidophilic and thermophilic obligate methanotroph, Methylacidiphilum infernorum. HGbI has very high affinity for O(2) and shows barely detectable autoxidation in the pH range of 5.2-8.6 and temperature range of 25-50°C. Examination of the heme pocket by X-ray crystallography and molecular dynamics showed that conformational movements of Tyr29(B10) and Gln50(E7), as well as structural flexibility of the GH loop and H-helix, may play a role in modulating its ligand binding behavior. Bacterial HGbI's unique resistance to the sort of extreme acidity that would extract heme from any other hemoglobin makes it an ideal candidate for comparative structure-function studies of the expanding globin superfamily.

PubMed: 21925500
DOI: 10.1016/j.febslet.2011.09.002

Experimental method

X-RAY DIFFRACTION (1.8 Å)