3RWL

Structure of P450pyr hydroxylase

3RWL の概要

• エントリーDOI: 10.2210/pdb3rwl/pdb
• 分子名称: Cytochrome P450 alkane hydroxylase 1 CYP153A7, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: p450 monooxygenase, oxidoreductase
• 由来する生物種: Sphingopyxis macrogoltabida
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48875.05

構造登録者

Pompidor, G. (登録日: 2011-05-09, 公開日: 2012-04-18, 最終更新日: 2024-02-28)

主引用文献

Pham, S.Q.,Pompidor, G.,Liu, J.,Li, X.D.,Li, Z.
Evolving P450pyr hydroxylase for highly enantioselective hydroxylation at non-activated carbon atom.
Chem.Commun.(Camb.), 48:4618-4620, 2012

PubMed Abstract: Directed evolution of a monooxygenase to achieve very high enantioselectivity for hydroxylation at non-activated carbon atoms is demonstrated for the first time, where a triple mutant of P450pyr hydroxylase is obtained via determination of enzyme structure, iterative saturation mutagenesis, and high-throughput screening with a MS-based ee assay to increase the product ee from 53% to 98% for the hydroxylation of N-benzyl pyrrolidine to (S)-N-benzyl 3-hydroxypyrrolidine.

PubMed: 22430002
DOI: 10.1039/c2cc30779k

実験手法

X-RAY DIFFRACTION (2 Å)