3RWK

First crystal structure of an endo-inulinase, from Aspergillus ficuum: structural analysis and comparison with other GH32 enzymes.

3RWK の概要

• エントリーDOI: 10.2210/pdb3rwk/pdb
• 分子名称: Inulinase, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: endo-inulinase, glycoside hydrolase family 32, catalytic mechanism, glycosidase hydrolase family 32, glycosylation, cytosol, hydrolase
• 由来する生物種: Aspergillus ficuum
• 細胞内の位置: Secreted: O94220
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 57460.96

構造登録者

Michaux, C.,Pouyez, J.,Roussel, G.,Mayard, A.,Vandamme, A.M.,Housen, I.,Wouters, J. (登録日: 2011-05-09, 公開日: 2012-07-18, 最終更新日: 2024-10-16)

• 主引用文献: Pouyez, J.,Mayard, A.,Vandamme, A.M.,Roussel, G.,Perpete, E.A.,Wouters, J.,Housen, I.,Michaux, C.; First crystal structure of an endo-inulinase, INU2, from Aspergillus ficuum: Discovery of an extra-pocket in the catalytic domain responsible for its endo-activity.; Biochimie, 94:2423-2430, 2012; PubMed: 22750808; DOI: 10.1016/j.biochi.2012.06.020

実験手法

X-RAY DIFFRACTION (2.1 Å)