3RW6

Structure of nuclear RNA export factor TAP bound to CTE RNA

3RW6 の概要

• エントリーDOI: 10.2210/pdb3rw6/pdb
• 分子名称: Nuclear RNA export factor 1, constitutive transport element(CTE)of Mason-Pfizer monkey virus RNA (3 entities in total)
• 機能のキーワード: retroviral constitutive transport element (cte), rna recognition motif (rrm), leucine-rich repeat (lrr) motif, transport protein-rna complex, transport protein/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus, nucleoplasm : Q9UBU9
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101589.79

構造登録者

Teplova, M.,Khin, N.W.,Patel, D.J.,Izaurralde, E. (登録日: 2011-05-07, 公開日: 2011-08-10, 最終更新日: 2023-09-13)

主引用文献

Teplova, M.,Wohlbold, L.,Khin, N.W.,Izaurralde, E.,Patel, D.J.
Structure-function studies of nucleocytoplasmic transport of retroviral genomic RNA by mRNA export factor TAP.
Nat.Struct.Mol.Biol., 18:990-998, 2011

PubMed Abstract: mRNA export is mediated by the TAP-p15 heterodimer, which belongs to the family of NTF2-like export receptors. TAP-p15 heterodimers also bind to the constitutive transport element (CTE) present in simian type D retroviral RNAs, and they mediate the export of viral unspliced RNAs to the host cytoplasm. We have solved the crystal structure of the RNA recognition and leucine-rich repeat motifs of TAP bound to one symmetrical half of the CTE RNA. L-shaped conformations of protein and RNA are involved in a mutual molecular embrace on complex formation. We have monitored the impact of structure-guided mutations on binding affinities in vitro and transport assays in vivo. Our studies define the principles by which CTE RNA subverts the mRNA export receptor TAP, thereby facilitating the nuclear export of viral genomic RNAs, and, more generally, provide insights on cargo RNA recognition by mRNA export receptors.

PubMed: 21822283
DOI: 10.1038/nsmb.2094

実験手法

X-RAY DIFFRACTION (2.3 Å)