3RUI

Crystal structure of Atg7C-Atg8 complex

3RUI の概要

• エントリーDOI: 10.2210/pdb3rui/pdb
• 関連するPDBエントリー: 2ZPN 3RUJ
• 分子名称: Ubiquitin-like modifier-activating enzyme ATG7, Autophagy-related protein 8, ZINC ION, ... (4 entities in total)
• 機能のキーワード: autophagy, autophagosome formation, non-canonical e1, atp binding, ubl, atg8, atg12, atg10, atg3, ubl activation, thiolation, zinc finger, gxgxxg motif atp binding motif, e1-like protein, ligase, zinc binding, atg8 protein binding
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm: P38862; Cytoplasmic vesicle, cvt vesicle membrane; Lipid-anchor: P38182
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52143.93

構造登録者

Hong, S.B.,Kim, B.W.,Song, H.K. (登録日: 2011-05-05, 公開日: 2011-11-23, 最終更新日: 2024-10-30)

主引用文献

Hong, S.B.,Kim, B.W.,Lee, K.E.,Kim, S.W.,Jeon, H.,Kim, J.,Song, H.K.
Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8.
Nat.Struct.Mol.Biol., 18:1323-1330, 2011

PubMed Abstract: Autophagy is the degradation of cellular organelles via the lysosomal pathway. The autophagic ubiquitin-like (Ubl) molecule Atg8 is activated by the E1-like enzyme Atg7. As this noncanonical E1 enzyme's domain organization is unique among Ubl-activating E1 enzymes, the structural basis for its interactions with Atg8 and partner E2 enzymes remains obscure. Here we present the structure of the N-terminal domain of Atg7, revealing a unique protein fold and interactions with both autophagic E2 enzymes Atg3 and Atg10. The structure of the C-terminal domain of Atg7 in complex with Atg8 shows the mode of dimerization and mechanism of recognition of Atg8. Notably, the catalytic cysteine residue in Atg7 is positioned close to the C-terminal glycine of Atg8, its target for thioester formation, potentially eliminating the need for large conformational rearrangements characteristic of other E1s.

PubMed: 22056771
DOI: 10.1038/nsmb.2165

実験手法

X-RAY DIFFRACTION (1.906 Å)